IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Vimentin antibody . Western blot was performed from the immunoprecipitate using Vimentin antibody at a dilition of 1:1000.)
Rabbit Vimentin Monoclonal Antibody | anti-VIM antibody
[KO Validated] Vimentin Rabbit mAb
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:200
Customer Validation:
WB: Human, Mouse
IF: Mouse
IHC: Human
IF: Mouse
IF: Human
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Vimentin antibody . Western blot was performed from the immunoprecipitate using Vimentin antibody at a dilition of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Vimentin antibody . Western blot was performed from the immunoprecipitate using Vimentin antibody at a dilition of 1:1000.)
IF (Immunofluorescence)
(Confocal Immunofluorescent analysis of U2OS cells using KRR1 Rabbit pAb (A4487) at dilution of 1:100 (40x lens)(red). Vimentin (: Vimentin Rabbit mAb) for Cytoskeleton staining(green), DAPI for nuclear staining(blue).)
IF (Immunofluorescence)
(Confocal Immunofluorescent analysis of U2OS cells using KRR1 Rabbit pAb (A4487) at dilution of 1:100 (40x lens)(red). Vimentin (: Vimentin Rabbit mAb) for Cytoskeleton staining(green), DAPI for nuclear staining(blue).)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Mouse kidney using Vimentin antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Mouse kidney using Vimentin antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Human colon carcinoma using Vimentin antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Human colon carcinoma using Vimentin antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Rat spleen using Vimentin antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Rat spleen using Vimentin antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Vimentin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Vimentin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
NCBI and Uniprot Product Information
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Product Notes
The VIM vim (Catalog #AAA28380) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The [KO Validated] Vimentin Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Vimentin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200 IP: 1:50-1:200 Customer Validation: WB: Human, Mouse IF: Mouse IHC: Human IF: Mouse IF: Human. Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vimentin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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