IP (Immunoprecipitation)
(Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug N-Cadherin antibody. Western blot was performed from the immunoprecipitate using N-Cadherin antibody at a dilution of 1:1000.)
Rabbit N-Cadherin Polyclonal Antibody | anti-LRRC59 antibody
[KO Validated] N-Cadherin Rabbit pAb
IHC: 1:50-1:200
IP: 1:50-1:200
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug N-Cadherin antibody. Western blot was performed from the immunoprecipitate using N-Cadherin antibody at a dilution of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug N-Cadherin antibody. Western blot was performed from the immunoprecipitate using N-Cadherin antibody at a dilution of 1:1000.)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Mouse liver using N-Cadherin antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Mouse liver using N-Cadherin antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Human colon carcinoma using N-Cadherin antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Human colon carcinoma using N-Cadherin antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of extracts of 293T cells, using N-Cadherin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of extracts of 293T cells, using N-Cadherin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using N-Cadherin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using N-Cadherin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using N-Cadherin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using N-Cadherin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
NCBI and Uniprot Product Information
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Product Notes
The LRRC59 lrrc59 (Catalog #AAA28412) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The [KO Validated] N-Cadherin Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's N-Cadherin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 IP: 1:50-1:200. Researchers should empirically determine the suitability of the LRRC59 lrrc59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MTKAGSKGGN LRDKLDGNEL DLSLSDLNEV PVKELAALPK ATILDLSCNK LTTLPSDFCG LTHLVKLDLS KNKLQQLPAD FGRLVNLQHL DLLNNKLVTL PVSFAQLKNL KWLDLKDNPL DPVLAKVAGD CLDEKQCKQC ANKVLQHMKA VQADQERERQ RRLEVEREAE KKREAKQRAK EAQERELRKR EKAEEKERRR KEYDALKAAK REQEKKPKKE ANQAPKSKSG SRPRKPPPRK HTRS. It is sometimes possible for the material contained within the vial of "N-Cadherin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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