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FCM (Flow Cytometry)
(ID1 Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ID1 Polyclonal Antibody | anti-ID1 antibody
ID1 Antibody (Center)
Gene Names
ID1; ID; bHLHb24
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ID1; Polyclonal Antibody; ID1 Antibody (Center); DNA-binding protein inhibitor ID-1; Class B basic helix-loop-helix protein 24; bHLHb24; Inhibitor of DNA binding 1; Inhibitor of differentiation 1; BHLHB24; ID; anti-ID1 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the Central region of human ID1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.26 (varies by lot)
Sequence Positions
66-93
Sequence Length
155
Applicable Applications for anti-ID1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM (Flow Cytometry)
(ID1 Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM (Flow Cytometry)
(ID1 Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ID1 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ID1 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemistry)
(ID1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ID1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(ID1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ID1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ID1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ID1(PEI 1:100) antibody detected the ID1 protein (arrow).)
WB (Western Blot)
(ID1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ID1(PEI 1:100) antibody detected the ID1 protein (arrow).)
WB (Western Blot)
(ID1 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ID1 antibody detected the ID1 protein (arrow).)
WB (Western Blot)
(ID1 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ID1 antibody detected the ID1 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from HepG2, MCF-7, U-2OS cell line (from left to right), using ID1 Antibody (Center). AAA28652 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from HepG2, MCF-7, U-2OS cell line (from left to right), using ID1 Antibody (Center). AAA28652 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-ID1 antibody
The protein encoded by this gene is a helix-loop-helix
(HLH) protein that can form heterodimers with members of the basic
HLH family of transcription factors. The encoded protein has no DNA
binding activity and therefore can inhibit the DNA binding and
transcriptional activation ability of basic HLH proteins with which
it interacts. This protein may play a role in cell growth,
senescence, and differentiation. Two transcript variants encoding
different isoforms have been found for this gene. [provided by
RefSeq].
(HLH) protein that can form heterodimers with members of the basic
HLH family of transcription factors. The encoded protein has no DNA
binding activity and therefore can inhibit the DNA binding and
transcriptional activation ability of basic HLH proteins with which
it interacts. This protein may play a role in cell growth,
senescence, and differentiation. Two transcript variants encoding
different isoforms have been found for this gene. [provided by
RefSeq].
Product Categories/Family for anti-ID1 antibody
References
Qian, T., et al. Oncogene 29(43):5818-5827(2010)
Hamajima, Y., et al. Cell Prolif. 43(5):457-463(2010)
Yang, J., et al. Circ. Res. 107(2):252-262(2010)
Phi, J.H., et al. J Neurosurg Pediatr 5(6):608-614(2010)
Geng, H., et al. Cancer Res. 70(8):3239-3248(2010)
Hamajima, Y., et al. Cell Prolif. 43(5):457-463(2010)
Yang, J., et al. Circ. Res. 107(2):252-262(2010)
Phi, J.H., et al. J Neurosurg Pediatr 5(6):608-614(2010)
Geng, H., et al. Cancer Res. 70(8):3239-3248(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16133
NCBI Official Full Name
DNA-binding protein inhibitor ID-1 isoform a
NCBI Official Synonym Full Names
inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
NCBI Official Symbol
ID1
NCBI Official Synonym Symbols
ID; bHLHb24
NCBI Protein Information
DNA-binding protein inhibitor ID-1
UniProt Protein Name
DNA-binding protein inhibitor ID-1
UniProt Gene Name
ID1
UniProt Synonym Gene Names
BHLHB24; ID; bHLHb24
UniProt Entry Name
ID1_HUMAN
Similar Products
Product Notes
The ID1 id1 (Catalog #AAA28652) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 66-93. The ID1 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ID1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the ID1 id1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ID1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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