Mouse alpha-tubulin Monoclonal Antibody | anti-TUBA1B antibody

alpha-tubulin (Acetyl Lys40) Monoclonal Antibody (4A8)

Cat. Num. AAA30827

• Gene Names: TUBA1B; K-ALPHA-1
• Reactivity: Human, Rat, Mouse, Drosophila
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Synonyms: alpha-tubulin; Monoclonal Antibody; alpha-tubulin (Acetyl Lys40) Monoclonal Antibody (4A8); Tubulin alpha-1B chain (Alpha-tubulin ubiquitous)(Tubulin K-alpha-1)(Tubulin alpha-ubiquitous chain); anti-TUBA1B antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse, Drosophila
• Clonality: Monoclonal
• Clone Number: 4A8
• Specificity: alpha-tubulin (Acetyl Lys40) protein detects endogenous levels of alpha-tubulin (Acetyl Lys40)
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TUBA1B antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:1000-2000; IHC: 1:50-100; IF: 1:200
• Immunogen: Synthetic Peptide of alpha-tubulin (Acetyl Lys40)
• Modification: Acetyl Specific
• Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
• PTM: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.; Belongs to the tubulin family.
• Subunit: Dimer of alpha and beta chains.
• Preparation and Storage: Store at -20 degree C/1 year

IF (Immunofluorescence)

(Immunofluorescence analysis of Rat-spinal-cord tissue. 1.?-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Mouse-lung tissue. 1.?-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Human-liver-cancer tissue. 1.?-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.?-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1.?-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using a-tubulin(Acetyl Lys40) Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.?-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using a-tubulin(Acetyl Lys40) Mouse mAb diluted at 1:200.)

WB (Western Blot)

(Western blot analysis of extracts from Hela cells. untreated (-) or treated with TSA (1 uM. 18 hr; +). using Acetyl- a-tubulin(Lys40) Mouse mAb 1:2000.)

Product Categories/Family for anti-TUBA1B antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 55977474
• NCBI GeneID: 10376
• UniProt Accession #: P68363; P04687; P05209; Q27I68; Q8WU19
• Molecular Weight: 37,218 Da
• NCBI Official Full Name: Tubulin alpha-1B chain
• NCBI Official Synonym Full Names: tubulin, alpha 1b
• NCBI Official Symbol: TUBA1B
• NCBI Official Synonym Symbols: K-ALPHA-1
• NCBI Protein Information: tubulin alpha-1B chain; alpha tubulin; tubulin K-alpha-1; alpha-tubulin ubiquitous; tubulin, alpha, ubiquitous; tubulin alpha-ubiquitous chain
• UniProt Protein Name: Tubulin alpha-1B chain
• UniProt Gene Name: TUBA1B
• UniProt Entry Name: TBA1B_HUMAN