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IF (Immunofluorescence)
(Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Rabbit CD38 Polyclonal Antibody | anti-CD38 antibody
CD38 Antibody (C-term)
Gene Names
CD38; T10; ADPRC 1
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CD38; Polyclonal Antibody; CD38 Antibody (C-term); ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADPRC 1; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; anti-CD38 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CD38 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-270 amino acids from the C-terminal region of human CD38.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
241-270
Sequence Length
122
Applicable Applications for anti-CD38 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
IF (Immunofluorescence)
(Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD38 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD38 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells using CD38 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells using CD38 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Immunofluorescence analysis of CD38 Antibody (C-term) with paraffin-embedded human hepatocarcinoma tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
IF (Immunofluorescence)
(Immunofluorescence analysis of CD38 Antibody (C-term) with paraffin-embedded human hepatocarcinoma tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD38 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD38 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysate from RPMI8226 cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from RPMI8226 cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from 293T cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from 293T cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Related Product Information for anti-CD38 antibody
CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling.
Product Categories/Family for anti-CD38 antibody
References
Horenstein,A.L., Mol. Med. 15 (3-4), 76-84 (2009) Liu,Q., Biochemistry 47 (52), 13966-13973 (2008) Liu,Q., Chem. Biol. 15 (10), 1068-1078 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34328
NCBI Official Full Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
NCBI Official Synonym Full Names
CD38 molecule
NCBI Official Symbol
CD38
NCBI Official Synonym Symbols
T10; ADPRC 1
NCBI Protein Information
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
UniProt Protein Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
UniProt Gene Name
CD38
UniProt Synonym Gene Names
ADPRC 1; cADPr hydrolase 1
UniProt Entry Name
CD38_HUMAN
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Product Notes
The CD38 cd38 (Catalog #AAA28702) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 241-270. The CD38 Antibody (C-term) reacts with Human (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's CD38 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the CD38 cd38 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD38, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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