Rabbit anti-Human GAPDH Polyclonal Antibody | anti-GAPDH antibody
GAPDH Antibody (N-term)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(GAPDH Antibody (N-term) confocal immunofluorescent analysis with Hela cell. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of GAPDH Antibody (N-term) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GAPDH Antibody (N-term). AAA28776 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from A375 cell line, using GAPDH Antibody (N-term). AAA28776 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Lu,J., Biosci. Biotechnol. Biochem. 72 (9), 2432-2435 (2008)
Zhou,Y., Mol. Cancer Res. 6 (8), 1375-1384 (2008)
