Rabbit anti-Human NOS2A Polyclonal Antibody | anti-NOS2 antibody

NOS2A Antibody (Center)

Cat. Num. AAA28780

• Gene Names: NOS2; NOS; INOS; NOS2A; HEP-NOS
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: NOS2A, Antibody; NOS2A Antibody (Center); Nitric oxide synthase; inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; NOS2A; anti-NOS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This NOS2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 830-860 amino acids from the Central region of human NOS2A.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 830-860
• Sequence Length: 1,114

• Applicable Applications for anti-NOS2 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of NOS2A Antibody (Center) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

FCM (Flow Cytometry)

(NOS2A Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry)

(NOS2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NOS2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(NOS2A Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NOS2A antibody detected the NOS2A protein (arrow).)

WB (Western Blot)

(Anti-NOS2A Antibody (Center)at 1:2000 dilution + A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 131 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-NOS2A Antibody (Center)at 1:2000 dilution + Y79 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 131 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NOS2 antibody

Nitric oxide is a reactive free radical which acts as a
biologic mediator in several processes, including neurotransmission
and antimicrobial and antitumoral activities. This gene encodes a
nitric oxide synthase which is expressed in liver and is inducible
by a combination of lipopolysaccharide and certain cytokines. Three
related pseudogenes are located within the Smith-Magenis syndrome
region on chromosome 17.

Product Categories/Family for anti-NOS2 antibody

Cancer; Metabolism; Neuroscience; Signal transduction

References

Ryk, C., et al. J. Urol. 184(5):2150-2157(2010)
Planche, T., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 299 (5), R1248-R1253 (2010) :
Feng, C., et al. FEBS Lett. 584(20):4335-4338(2010)
Mokrzycka, M., et al. Folia Histochem. Cytobiol. 48(2):191-196(2010)
Tupitsyna, T.V., et al. Mol. Gen. Mikrobiol. Virusol. 3, 3-7 (2010) :

NCBI and Uniprot Product Information

• NCBI GI #: 24041029
• NCBI GeneID: 4843
• NCBI Accession #: NP_000616.3
• NCBI GenBank Nucleotide #: NM_000625.4
• UniProt Accession #: P35228; O60757; O94994; Q16263; Q16692; Q4TTS5; Q9UD42; A1L3U5; B7ZLY2
• Molecular Weight: 131117
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: NOS; INOS; NOS2A; HEP-NOS
• NCBI Protein Information: nitric oxide synthase, inducible
• UniProt Protein Name: Nitric oxide synthase, inducible
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: NOS2A; HEP-NOS; Inducible NOS; iNOS
• UniProt Entry Name: NOS2_HUMAN