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IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
Rabbit anti-Human, Mouse Cleaved-PARP-1 Polyclonal Antibody | anti-PARP1 antibody
Cleaved-PARP-1 (D214) Polyclonal Antibody
Gene Names
PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Synonyms
Cleaved-PARP-1; Polyclonal Antibody; Cleaved-PARP-1 (D214) Polyclonal Antibody; PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD (+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1; anti-PARP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Applicable Applications for anti-PARP1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
IHC (Immunohistchemistry)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
IHC (Immunohistchemistry)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
IHC (Immunohistochemistry-Paraffin)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
IHC (Immunohistochemistry-Paraffin)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
IHC (Immunohistochemistry-Paraffin)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
IHC (Immunohistochemistry-Paraffin)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
IHC (Immunohistochemistry-Paraffin)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
IHC (Immunohistochemistry-Paraffin)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
WB (Western Blot)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
WB (Western Blot)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
Related Product Information for anti-PARP1 antibody
Poly-ADP-ribosyltransferase that mediates poly-ADP- ribosylation of proteins and plays a key role in DNA repair (PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272, PubMed:25043379, PubMed:26344098). Mainly mediates glutamate and aspartate ADP- ribosylation of target proteins: the ADP-D-ribosyl group of NAD (+) is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (PubMed:9315851, PubMed:19764761, PubMed:25043379). Mediates the poly (ADP-ribosyl)ation of a number of proteins, including itself, APLF and CHFR (PubMed:19764761). Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). In addition to base excision repair (BER) pathway, also involved in double-strand breaks (DSBs) repair: together with TIMELESS, accumulates at DNA damage sites and promotes homologous recombination repair by mediating poly-ADP- ribosylation (PubMed:30356214). In addition to proteins, also able to ADP-ribosylate DNA: catalyzes ADP- ribosylation of DNA strand break termini containing terminal phosphates and a 2'-OH group in single- and double-stranded DNA, respectively (PubMed:27471034). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). Acts as a regulator of transcription: positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150 (PubMed:19344625). With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
113,084 Da
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly (ADP-ribose) polymerase 1
NCBI Official Symbol
PARP1
NCBI Official Synonym Symbols
PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
NCBI Protein Information
poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-
UniProt Protein Name
Poly [ADP-ribose] polymerase 1
UniProt Gene Name
PARP1
UniProt Synonym Gene Names
ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1
UniProt Entry Name
PARP1_HUMAN
Similar Products
Product Notes
The PARP1 parp1 (Catalog #AAA28838) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cleaved-PARP-1 (D214) Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cleaved-PARP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF). Researchers should empirically determine the suitability of the PARP1 parp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cleaved-PARP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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