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IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cell using PIN1 antibody. Blue: DAPI for nuclear staining.)
Rabbit PIN1 Polyclonal Antibody | anti-PIN1 antibody
PIN1 Antibody
Gene Names
PIN1; DOD; UBL5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
PIN1; Polyclonal Antibody; PIN1 Antibody; DOD; UBL5; anti-PIN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total PIN1 protein.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
163
Applicable Applications for anti-PIN1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:200
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:200
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human PIN1.
Target Name
PIN1
Preparation and Storage
Store at -20 degree C
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cell using PIN1 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cell using PIN1 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat spleen using PIN1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat spleen using PIN1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat lung using PIN1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat lung using PIN1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat kidney using PIN1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat kidney using PIN1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human esophageal cancer using PIN1 antibody at dilution of 1:100 (400x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human esophageal cancer using PIN1 antibody at dilution of 1:100 (400x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PIN1 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PIN1 antibody.)
Related Product Information for anti-PIN1 antibody
Pin1, a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2/M transition of the mammalian cell cycle (1-6). Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).
Product Categories/Family for anti-PIN1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,243 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
NCBI Official Synonym Full Names
peptidylprolyl cis/trans isomerase, NIMA-interacting 1
NCBI Official Symbol
PIN1
NCBI Official Synonym Symbols
DOD; UBL5
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
UniProt Gene Name
PIN1
UniProt Synonym Gene Names
PPIase Pin1
UniProt Entry Name
PIN1_HUMAN
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Product Notes
The PIN1 pin1 (Catalog #AAA29684) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIN1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PIN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the PIN1 pin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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