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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with AOF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Mouse anti-Human AOF1 Monoclonal Antibody | anti-AOF1 antibody
AOF1 Antibody
Gene Names
KDM1B; AOF1; LSD2; C6orf193; bA204B7.3; dJ298J15.2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
AOF1; Monoclonal Antibody; AOF1 Antibody; amine oxidase (flavin containing) domain 1 antibody; amine oxidase; flavin containing 1 antibody; AOF1 antibody; bA204B7.3 antibody; C6orf193 antibody; dJ298J15.2 antibody; Flavin-containing amine oxidase domain-containing protein 1 antibody; FLJ33898 antibody; FLJ34109 antibody; FLJ43328 antibody; KDM1B antibody; KDM1B_HUMAN antibody; LSD2 antibody; lysine (K)-specific demethylase 1B antibody; Lysine-specific demethylase 2 antibody; Lysine-specific histone demethylase 1B antibody; Lysine-specific histone demethylase 2 antibody; anti-AOF1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-AOF1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:100-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:100-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with AOF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with AOF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry)
(ICC staining AOF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining AOF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of AOF1 on HEK293 (1) and AOF1-hIgGFc transfected HEK293 (2) cell lysate using anti-AOF1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of AOF1 on HEK293 (1) and AOF1-hIgGFc transfected HEK293 (2) cell lysate using anti-AOF1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of AOF1 on human AOF1 recombinant protein using anti-AOF1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of AOF1 on human AOF1 recombinant protein using anti-AOF1 antibody at 1/1, 000 dilution.)
Related Product Information for anti-AOF1 antibody
AOF1 (amine-oxidase flavin-containing domain 1), whose alternative names include KDM1B (lysine (K)-specific demethylase 1B) or LSD2 (lysine-specific histone demethylase 2), is an 823 amino acid nuclear protein belonging to the flavin monoamine oxidase family. As a histone demethylase, AOF1 specifically demethylates Lys4 of histone H3, a marker for epigenetic transcriptional activation, by interacting with a long stretch of the H3 N-terminal tail of mono- and dimethylated Lys4. Existing as three alternatively spliced isoforms, AOF1 is required for de novo DNA methylation of some imprinted genes in oocytes and contains a single SWIRM domain that is implicated in chromatin regulation. AOF1 contains one CW-type zinc finger and is encoded by a gene located on human chromosome 6p22.3.
Product Categories/Family for anti-AOF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 65 kDa
Observed: 68 kDa
Observed: 68 kDa
NCBI Official Full Name
lysine-specific histone demethylase 1B
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 1B
NCBI Official Symbol
KDM1B
NCBI Official Synonym Symbols
AOF1; LSD2; C6orf193; bA204B7.3; dJ298J15.2
NCBI Protein Information
lysine-specific histone demethylase 1B; amine oxidase, flavin containing 1; lysine-specific histone demethylase 2; amine oxidase (flavin containing) domain 1; flavin-containing amine oxidase domain-containing protein 1
UniProt Protein Name
Lysine-specific histone demethylase 1B
UniProt Gene Name
KDM1B
UniProt Synonym Gene Names
AOF1; C6orf193; LSD2
UniProt Entry Name
KDM1B_HUMAN
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Product Notes
The AOF1 kdm1b (Catalog #AAA29903) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AOF1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AOF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the AOF1 kdm1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AOF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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