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ICC (Immunocytochemistry)
(ICC staining Synaptophysin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit Synaptophysin Monoclonal Antibody | anti-SYP antibody
Synaptophysin Antibody
Gene Names
SYP; MRXSYP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
Synaptophysin; Monoclonal Antibody; Synaptophysin Antibody; Major synaptic vesicle protein p38 antibody; MRX96 antibody; MRXSYP antibody; Syn p38 antibody; Synaptophysin antibody; Syp antibody; SYPH antibody; SYPH_HUMAN antibody; SypI antibody; anti-SYP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SJ26-85
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-SYP antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-5000
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining Synaptophysin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Synaptophysin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Synaptophysin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Synaptophysin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution. Positive control�� <br/>Lane 1: Human brain <br/>Lane 2: PC-12)
WB (Western Blot)
(Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution. Positive control�� <br/>Lane 1: Human brain <br/>Lane 2: PC-12)
Related Product Information for anti-SYP antibody
Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis. In addition, the tail domain of brain Myosin V also forms a stable complex with synaptobrevin II and SYP, and this complex is disassembled upon the depolarization-induced entry of Ca2+ into intact nerve endings.
Product Categories/Family for anti-SYP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,845 Da
NCBI Official Full Name
synaptophysin
NCBI Official Synonym Full Names
synaptophysin
NCBI Official Symbol
SYP
NCBI Official Synonym Symbols
MRXSYP
NCBI Protein Information
synaptophysin; major synaptic vesicle protein P38
UniProt Protein Name
Synaptophysin
UniProt Gene Name
SYP
UniProt Entry Name
SYPH_HUMAN
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Product Notes
The SYP syp (Catalog #AAA30051) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Synaptophysin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Synaptophysin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000-5000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the SYP syp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Synaptophysin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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