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ICC (Immunocytochemistry)
(ICC staining Dysferlin in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit anti-Human, Mouse Dysferlin Monoclonal Antibody | anti-DYSF antibody
Dysferlin Antibody
Gene Names
DYSF; MMD1; FER1L1; LGMD2B
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
Dysferlin; Monoclonal Antibody; Dysferlin Antibody; DMAT antibody; DYSF antibody; DYSF_HUMAN antibody; Dysferlin antibody; Dysferlin limb girdle muscular dystrophy 2B (autosomal recessive) antibody; Dysferlin limb girdle muscular dystrophy 2B antibody; Dystrophy associated fer 1 like 1 antibody; Dystrophy associated fer 1 like protein antibody; Dystrophy associated fer1 like 1 antibody; Dystrophy associated fer1 like protein antibody; Dystrophy-associated fer-1-like protein antibody; Fer 1 like protein 1 antibody; Fer-1-like protein 1 antibody; Fer1 like protein 1 antibody; FER1L1 antibody; FLJ00175 antibody; FLJ90168 antibody; LGMD 2B antibody; LGMD2B antibody; Limb girdle muscular dystrophy 2B (autosomal recessive) antibody; Limb girdle muscular dystrophy 2B antibody; Miyoshi myopathy antibody; MM antibody; MMD1 antibody; anti-DYSF antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
JF05-54
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-DYSF antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-5000
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining Dysferlin in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Dysferlin in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Dysferlin on human skeletal muscle lysates using anti-Dysferlin antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of Dysferlin on human skeletal muscle lysates using anti-Dysferlin antibody at 1/1, 000 dilution.)
Related Product Information for anti-DYSF antibody
Dysferlin is a muscle-specific protein that is essential for normal muscle function and development. Mutations in the human dysferlin gene, DYSF, which maps to chromosome 2p13.2, are associated with limb girdle muscular dystrophy-2B (LGMD-2B) and a related, adult-onset, distal dystrophy known as Miyoshi myopathy (MM). Dysferlin localizes to the muscle fiber membrane, but is absent in MM and LGMD-2B muscle. Dysferlin is detected in 5-6 week embryos, when limbs begin to form regional differentiation. Although it is not essential for initial myogenesis, dysferlin appears to be critical for sustained normal function in mature muscle. It has been suggested that the absence of dysferlin during development gives rise to the disease phenotype in adulthood. Identical mutations in the dysferlin gene can produce more than one myopathy phenotype, indicating that additional genes and/or other factors are also involved in the clinical phenotype. The DYSF gene has no homology to any other known mammalian gene, but the protein product is related to the spermatogenesis factor fer-1 of Caenorhabditis elegans. The name dysferlin combines the role of the gene in producing muscular dystrophy with its homology to C. elegans.
Product Categories/Family for anti-DYSF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
237,295 Da
NCBI Official Full Name
dysferlin isoform 2
NCBI Official Synonym Full Names
dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive)
NCBI Official Symbol
DYSF
NCBI Official Synonym Symbols
MMD1; FER1L1; LGMD2B
NCBI Protein Information
dysferlin; fer-1-like protein 1; dystrophy-associated fer-1-like 1; dystrophy-associated fer-1-like protein
UniProt Protein Name
Dysferlin
UniProt Gene Name
DYSF
UniProt Synonym Gene Names
FER1L1
UniProt Entry Name
DYSF_HUMAN
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Product Notes
The DYSF dysf (Catalog #AAA30280) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Dysferlin Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Dysferlin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000-5000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the DYSF dysf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dysferlin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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