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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with ATP5A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit ATP5A1 Monoclonal Antibody | anti-ATP5A1 antibody
ATP5A1 Antibody
Gene Names
ATP5A1; OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
ATP5A1; Monoclonal Antibody; ATP5A1 Antibody; ATP synthase alpha chain; mitochondrial antibody; ATP synthase subunit alpha antibody; ATP synthase subunit alpha mitochondrial antibody; ATP synthase; H+ transporting; mitochondrial F1 complex; alpha subunit 1; cardiac muscle antibody; alpha subunit; 1 antibody; isoform 1; isoform 2; non-cardiac muscle-like 2 antibody; ATP sythase (F1 ATPase) alpha subunit antibody; ATP5A antibody; Atp5a1 antibody; ATP5AL2 antibody; ATPA_HUMAN antibody; ATPM antibody; Epididymis secretory sperm binding protein Li 123m antibody; hATP1 antibody; HEL-S-123m antibody; MC5DN4 antibody; Mitochondrial ATP synthetase antibody; Mitochondrial ATP synthetase oligomycin resistant antibody; Modifier of Min 2 mouse homolog antibody; Modifier of Min 2; mouse; homolog of antibody; MOM2 antibody; OMR antibody; ORM antibody; OTTHUMP00000163475 antibody; anti-ATP5A1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JM110-04
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-ATP5A1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-5000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with ATP5A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with ATP5A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining ATP5A1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining ATP5A1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining ATP5A1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining ATP5A1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining ATP5A1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining ATP5A1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart muscle tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart muscle tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of ATP5A1 on different cells lysates using anti-ATP5A1 antibody at 1/500 dilution. Positive control�� Line 1: Hela Line 2: MCF-7)
WB (Western Blot)
(Western blot analysis of ATP5A1 on different cells lysates using anti-ATP5A1 antibody at 1/500 dilution. Positive control�� Line 1: Hela Line 2: MCF-7)
Related Product Information for anti-ATP5A1 antibody
Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three alpha chains and three beta chains, which are weakly homologous, as well as one gamma chain, one delta chain and one e chain. F0 consists of three subunits: a, b and c. The alpha chain of F1 is a regulatory subunit that contains 509 amino acids. Mitochondrial ATPase alpha chain (ATP5A) localizes to the mitochondria and catalyzes ATP synthesis.
Product Categories/Family for anti-ATP5A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,751 Da
NCBI Official Full Name
ATP synthase subunit alpha, mitochondrial isoform c
NCBI Official Synonym Full Names
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
NCBI Official Symbol
ATP5A1
NCBI Official Synonym Symbols
OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2
NCBI Protein Information
ATP synthase subunit alpha, mitochondrial; ATP sythase (F1-ATPase) alpha subunit; ATP synthase alpha chain, mitochondrial; mitochondrial ATP synthetase, oligomycin-resistant; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform
UniProt Protein Name
ATP synthase subunit alpha, mitochondrial
UniProt Gene Name
ATP5A1
UniProt Synonym Gene Names
ATP5A; ATP5AL2; ATPM
UniProt Entry Name
ATPA_HUMAN
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Product Notes
The ATP5A1 atp5a1 (Catalog #AAA30323) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP5A1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATP5A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-5000 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the ATP5A1 atp5a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP5A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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