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ICC (Immunocytochemistry)
(ICC staining RAC1/2/3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit Rac1-2-3 Monoclonal Antibody | anti-RAC1 antibody
Rac1-2-3 Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
Rac1-2-3; Monoclonal Antibody; Rac1-2-3 Antibody; Cell migration inducing gene 5 protein antibody; EN 7 antibody; OTTMUSP00000004488 antibody; p21 Rac3 antibody; p21-Rac3 antibody; Rac1B antibody; RAC3 antibody; RAC3_HUMAN antibody; RAS related C3 botulinum substrate 3 antibody; Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) antibody; Ras related C3 botulinum toxin substrate 3 (rho family; small GTP binding protein Rac3) antibody; Ras-related C3 botulinum toxin substrate 3 antibody; Rho family small GTP binding protein Rac3 antibody; RP23-84C12.18 antibody; EN-7 antibody; EN7 antibody; GX antibody; HSPC 022 antibody; HSPC022 antibody; p21 Rac 2 antibody; p21 Rac2 antibody; p21-Rac2 antibody; p21Rac2 antibody; RAC 2 antibody; Rac2 antibody; RAC2_HUMAN antibody; Ras related C3 botulinum toxin substrate 2 (rho family; small GTP binding protein Rac2 antibody; Ras related C3 botulinum toxin substrate 2 antibody; small GTP binding protein Rac2) antibody; Ras related C3 botulinum toxin substrate 3 antibody; Ras-related C3 botulinum toxin substrate 2 antibody; Rho family small GTP binding protein Rac 2 antibody; Rho family small GTP binding protein Rac2 antibody; Small G protein antibody; Cell migration-inducing gene 5 protein antibody; MGC111543 antibody; MIG5 antibody; Migration inducing gene 5 antibody; Migration inducing protein 5 antibody; p21 Rac1 antibody; p21-Rac1 antibody; Rac 1 antibody; RAC1 antibody; RAC1_HUMAN antibody; Ras like protein TC25 antibody; Ras related C3 botulinum toxin substrate 1 (rho family; small GTP binding protein Rac1) antibody; Ras-like protein TC25 antibody; Ras-related C3 botulinum toxin substrate 1 antibody; Rho family small GTP binding protein Rac1 antibody; TC 25 antibody; TC25 antibody; anti-RAC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JM11-29
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RAC1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining RAC1/2/3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RAC1/2/3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RAC1/2/3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RAC1/2/3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RAC1/2/3 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RAC1/2/3 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of RAC1/2/3 on MCF-7 cells lysates using anti-RAC1/2/3 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of RAC1/2/3 on MCF-7 cells lysates using anti-RAC1/2/3 antibody at 1/500 dilution.)
Related Product Information for anti-RAC1 antibody
Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
Product Categories/Family for anti-RAC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
192
NCBI Official Full Name
Ras-related C3 botulinum toxin substrate 1
NCBI Official Synonym Full Names
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
NCBI Official Symbol
RAC1
NCBI Official Synonym Symbols
MIG5; Rac-1; TC-25; p21-Rac1
NCBI Protein Information
ras-related C3 botulinum toxin substrate 1; ras-like protein TC25; cell migration-inducing gene 5 protein
UniProt Protein Name
Ras-related C3 botulinum toxin substrate 1
UniProt Gene Name
RAC1
UniProt Synonym Gene Names
TC25
UniProt Entry Name
RAC1_HUMAN
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Product Notes
The RAC1 rac1 (Catalog #AAA30334) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rac1-2-3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rac1-2-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the RAC1 rac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rac1-2-3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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