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IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Goat GAPDH Polyclonal Antibody | anti-GAPDH antibody
Goat anti-GAPDH (Internal) Antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
GAPDH; Polyclonal Antibody; Goat anti-GAPDH (Internal) Antibody; Goat Anti-GAPDH (Internal); Biotinylated Antibody; glyceraldehyde-3-phosphate dehydrogenase; HGNC: 4141; G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432; aging-associated gene 9 protein; glyceraldehyde 3-phosphate dehydrogenase; GAPDH antibody; glyceraldehyde-3-phosphate dehydrogenase antibody; HGNC: 4141 antibody; G3PD antibody; GAPD antibody; MGC88685 antibody; OTTHUMP00000174431 antibody; OTTHUMP00000174432 antibody; aging-associated gene 9 protein antibody; glyceraldehyde 3-phosphate dehydrogenase antibody; GAPDH (Internal); anti-GAPDH antibody
Host
Goat
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
GVNHEKYDNSLK
Sequence Length
335
Applicable Applications for anti-GAPDH antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA:
antibody detection limit dilution 1:16000.
Western blot:
Approx 37kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1). Recommended concentration: 0.03-0.1 ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human
IHC:
In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2ug/ml.
Immunofluorescence:
Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10ug/ml.
antibody detection limit dilution 1:16000.
Western blot:
Approx 37kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1). Recommended concentration: 0.03-0.1 ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human
IHC:
In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2ug/ml.
Immunofluorescence:
Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10ug/ml.
Immunogen
Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2; NP_001243728.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
WB (Western Blot)
((0.03ug/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminesce)
WB (Western Blot)
((0.03ug/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminesce)
WB (Western Blot)
((0.1ug/ml) staining of Mouse Liver (A) and (0.03ug/ml) Rat Heart (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence)
WB (Western Blot)
((0.1ug/ml) staining of Mouse Liver (A) and (0.03ug/ml) Rat Heart (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence)
WB (Western Blot)
((0.1ug/ml) staining of Human Liver (A), (0.03ug/ml) Testes (B) and Tonsil (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence)
WB (Western Blot)
((0.1ug/ml) staining of Human Liver (A), (0.03ug/ml) Testes (B) and Tonsil (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence)
IHC (Immunohistochemistry)
((20ug/ml) staining of PFA-perfused cryosection of Rat Liver. Antigen retrieval with citrate buffer pH 3 by microwave for 20min, Cy3 detection. Data obtained by Dr. I. Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.)
IHC (Immunohistochemistry)
((20ug/ml) staining of PFA-perfused cryosection of Rat Liver. Antigen retrieval with citrate buffer pH 3 by microwave for 20min, Cy3 detection. Data obtained by Dr. I. Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.)
IHC (Immunohistochemistry)
((2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
IHC (Immunohistochemistry)
((2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
WB (Western Blot)
((0.5ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was overnight. Detected by alkaline phosphatase (NBT/BCIP). Data provided by Dr. I. Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.)
WB (Western Blot)
((0.5ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was overnight. Detected by alkaline phosphatase (NBT/BCIP). Data provided by Dr. I. Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.)
WB (Western Blot)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
References
Chakir K, Daya SK, Aiba T, Tunin RS, Dimaano VL, Abraham TP, Jaques-Robinson KM, Lai EW, Pacak K, Zhu WZ, Xiao RP, Tomaselli GF, Kass DA.; Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy.; Circulation.2009 Mar 10;119(9): 1231-40.; Pubmed 19237665
* The goat polyclonal antibody used (in WB on Human lymphocytes (PBMC)) as described in this paper was manufactured by us: ; Schillace RV, Miller CL, Pisenti N, Grotzke JE, Swarbrick GM, Lewinsohn DM, Carr DW.; A-kinase anchoring in dendritic cells is required for antigen presentation.; PLoS One.2009;4(3): e4807.; Pubmed 19277197
* The goat polyclonal antibody used (in WB on Human lymphocytes (PBMC)) as described in this paper was manufactured by us: ; Schillace RV, Miller CL, Pisenti N, Grotzke JE, Swarbrick GM, Lewinsohn DM, Carr DW.; A-kinase anchoring in dendritic cells is required for antigen presentation.; PLoS One.2009;4(3): e4807.; Pubmed 19277197
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 1
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD; HEL-S-162eP
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
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Product Notes
The GAPDH gapdh (Catalog #AAA13657) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GAPDH (Internal) Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 37kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1). Recommended concentration: 0.03-0.1 ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human IHC: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10ug/ml. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GVNHEKYDNS LK. It is sometimes possible for the material contained within the vial of "GAPDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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