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WB (Western Blot)
(Western Blot analysis of HepG2 cells using SPTLC1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)
Rabbit SPTLC1 Polyclonal Antibody | anti-SPTLC1 antibody
SPTLC1 Rabbit Polyclonal Antibody
Gene Names
SPTLC1; HSN1; LBC1; LCB1; SPT1; SPTI; HSAN1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
SPTLC1; Polyclonal Antibody; SPTLC1 Rabbit Polyclonal Antibody; LCB1; Serine palmitoyltransferase 1; Long chain base biosynthesis protein 1; LCB 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1; BH6842; anti-SPTLC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
SPTLC1 Polyclonal Antibody detects endogenous levels of SPTLC1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SPTLC1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Application Notes
WB: 1/500-1/2000
IHC-p: 1:100-300
ELISA: 1/20000
IHC-p: 1:100-300
ELISA: 1/20000
Immunogen
Synthesized peptide derived from SPTLC1. at AA range: 411-460
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
WB (Western Blot)
(Western Blot analysis of HepG2 cells using SPTLC1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)
WB (Western Blot)
(Western Blot analysis of HepG2 cells using SPTLC1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)
WB (Western Blot)
(Western blot analysis of 293T SH-SY5Y lysis using SPTLC1 antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)
WB (Western Blot)
(Western blot analysis of 293T SH-SY5Y lysis using SPTLC1 antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100)
Related Product Information for anti-SPTLC1 antibody
This gene encodes a member of the class-II pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is the long chain base subunit 1 of serine palmitoyltransferase. Serine palmitoyltransferase converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate and is the key enzyme in sphingolipid biosynthesis. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. Pseudogenes of this gene have been defined on chromosomes 1, 6, 10, and 13. [provided by RefSeq, Jul 2013]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
473
NCBI Official Full Name
Serine palmitoyltransferase 1
NCBI Official Synonym Full Names
serine palmitoyltransferase, long chain base subunit 1
NCBI Official Symbol
SPTLC1
NCBI Official Synonym Symbols
HSN1; LBC1; LCB1; SPT1; SPTI; HSAN1
NCBI Protein Information
serine palmitoyltransferase 1; LCB 1; SPT 1; serine C-palmitoyltransferase; serine-palmitoyl-CoA transferase 1; long chain base biosynthesis protein 1
UniProt Protein Name
Serine palmitoyltransferase 1
UniProt Gene Name
SPTLC1
UniProt Synonym Gene Names
LCB1; LCB 1; SPT 1; SPT1
UniProt Entry Name
SPTC1_HUMAN
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Product Notes
The SPTLC1 sptlc1 (Catalog #AAA30758) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPTLC1 Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SPTLC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA). WB: 1/500-1/2000 IHC-p: 1:100-300 ELISA: 1/20000. Researchers should empirically determine the suitability of the SPTLC1 sptlc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPTLC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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