Mouse GFAP Monoclonal Antibody | anti-GFAP antibody

GFAP Monoclonal Antibody (5C8)

Cat. Num. AAA30807

• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Synonyms: GFAP; Monoclonal Antibody; GFAP Monoclonal Antibody (5C8); Glial fibrillary acidic protein; anti-GFAP antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Clone Number: 5C8
• Specificity: The antibody detects endogenous GFAP proteins.
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Form/Format: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Applicable Applications for anti-GFAP antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC),
• Application Notes: WB: 1:2000-5000; IF: 1:200; IHC: 1:50-300
• Immunogen: Synthetic Peptide of GFAP
• Modification: Unmodified/Total
• Preparation and Storage: Store at -20 degree C/1 year

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.GFAP Monoclonal Antibody(5C8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.GFAP Monoclonal Antibody(5C8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.GFAP Monoclonal Antibody(5C8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

Application Data

(Wang. Hao. et al. "Effects of altitude changes on mild?to?moderate closed?head injury in rats following acute high?altitude exposure." Experimental and therapeutic medicine 17.1 (2019): 847-856.)

WB (Western Blot)

(Western blot analysis of Rat Brain Tissue. diluted at 1:5000.)

WB (Western Blot)

(Western blot analysis of lysates from 1) Rat Brain Tissue. 2)HeLa. 3)A431. 4) PC12 cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) Tubulin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)

Related Product Information for anti-GFAP antibody

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Product Categories/Family for anti-GFAP antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 196115290
• NCBI GeneID: 2670
• NCBI Accession #: NP_001124491.1
• NCBI GenBank Nucleotide #: NM_001131019.2
• UniProt Accession #: P14136; Q53H98; Q5D055; Q6ZQS3; Q7Z5J6; Q7Z5J7; Q96KS4; Q96P18; Q9UFD0; B2RD44; D3DX59
• Molecular Weight: 49,880 Da
• NCBI Official Full Name: glial fibrillary acidic protein isoform 2
• NCBI Official Synonym Full Names: glial fibrillary acidic protein
• NCBI Official Symbol: GFAP
• NCBI Protein Information: glial fibrillary acidic protein
• UniProt Protein Name: Glial fibrillary acidic protein
• UniProt Gene Name: GFAP
• UniProt Synonym Gene Names: GFAP
• UniProt Entry Name: GFAP_HUMAN