Human Proenkephalin (PENK) ELISA Kit | PENK elisa kit

Human Proenkephalin (PENK) ELISA Kit

Cat. Num. AAA31490

• Gene Names: PENK; PE; PENK-A
• Reactivity: Human
• Synonyms: Proenkephalin (PENK); Human Proenkephalin (PENK) ELISA Kit; PENK; Enkephalin A; preproenkephalin; PENK elisa kit

• Reactivity: Human
• Sequence Length: 267
• Assay Type: Sandwich
• Samples: Human serum, cell culture supernates and other biological fluids
• Detection Range: 18.75 pg/ml - 300 pg/ml
• Sensitivity: < 5pg/ml

• Intra-assay Precision: Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
• Inter-assay Precision: Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
• Preparation and Storage: Store at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PENK elisa kit

Background: Preproenkephalin has 267 amino acids, as does proopiomelanocortin. Both genes contain 2 introns. In both, all the repeated enkephalin or melanotropin sequences are encoded by a single large exon (exon 3). Preproenkephalin mRNA encodes 4 copies of met-enkephalin, 2 copies of met-enkephalin extended sequences, and 1 copy of leu-enkephalin. Each copy is flanked by paired basic amino acids which are presumably recognized by the processing protease. The corresponding amino acid sequence shows that the precursor is 267 amino acids long and contains 6 interspersed Met-enkephalin sequences and 1 Leu-enkephalin sequence. The precursor does not contain the sequences of dynorphin, alpha-neo-endorphin or beta-endorphin.

Principle of the Assay: Human Proenkephalin (PENK) ELISA Kit employs a two-site sandwich ELISA to quantitate PENK in samples. An antibody specific for PENK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PENK present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human PENK detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of PENK bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 208879446
• NCBI GeneID: 5179
• NCBI Accession #: NP_001129162.1
• NCBI GenBank Nucleotide #: NP_001129162.1
• UniProt Accession #: P01210
• NCBI Official Full Name: proenkephalin-A preproprotein
• NCBI Official Synonym Full Names: proenkephalin
• NCBI Official Symbol: PENK
• NCBI Official Synonym Symbols: PE; PENK-A
• NCBI Protein Information: proenkephalin-A
• UniProt Protein Name: Proenkephalin-A
• UniProt Gene Name: PENK
• UniProt Synonym Gene Names: OGF
• UniProt Entry Name: PENK_HUMAN