Standard Curve (Sample)
Mouse Thioredoxin-Interacting Protein (TXNIP) ELISA Kit | TXNIP elisa kit
Mouse Thioredoxin-Interacting Protein (TXNIP) ELISA Kit
Gene Names
Txnip; THIF; Tbp-2; VDUP1; Hyplip1; AA682105; 1200008J08Rik
Reactivity
Mouse
Synonyms
Thioredoxin-Interacting Protein (TXNIP); Mouse Thioredoxin-Interacting Protein (TXNIP) ELISA Kit; TXNIP; EST01027; HHCPA78; THIF; VDUP1; thioredoxin binding protein 2; upregulated by 1; 25-dihydroxyvitamin D-3; TXNIP elisa kit
Reactivity
Mouse
Specificity
Mouse Thioredoxin-interacting protein (TXNIP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TXNIP. No significant cross-reactivity or interference between Mouse TXNIP and analogues was observed.
Sequence Length
397
Assay Type
Sandwich
Samples
Mouse serum, cell culture supernates and other biological fluids
Detection Range
15 pg/ml - 240 pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TXNIP elisa kit
Background: Exposure to vitamin D3 (1,25-dihydroxyvitamin D3) or phorbol ester induces the bipotent HL-60 cell promyelocytic leukemia cell line to differentiate into monocytes/macrophages, whereas retinoic acid and dimethylsulfoxide induce differentiation towards granulocytes. The differentiation is accompanied by regulation of MYC, FOS, FMS (CSF1R), and myeloblastin (PRTN3;). By differential screening of HL60 cell lines, Chen and DeLuca (1994) identified a cDNA encoding TXNIP, which they termed VDUP1. The deduced TXNIP protein has 391 amino acids. Ribonuclease protection analysis showed dramatically increased expression of TXNIP in response to vitamin D3 but not to phorbol ester. Chen and DeLuca (1994) concluded that TXNIP is not involved in the differentiation process.
Principle of the Assay: Mouse Thioredoxin-interacting protein (TXNIP) ELISA Kit employs a two-site sandwich ELISA to quantitate TXNIP in samples. An antibody specific for TXNIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TXNIP present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Mouse TXNIP detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of TXNIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Mouse Thioredoxin-interacting protein (TXNIP) ELISA Kit employs a two-site sandwich ELISA to quantitate TXNIP in samples. An antibody specific for TXNIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TXNIP present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Mouse TXNIP detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of TXNIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thioredoxin-interacting protein isoform 1
NCBI Official Synonym Full Names
thioredoxin interacting protein
NCBI Official Symbol
Txnip
NCBI Official Synonym Symbols
THIF; Tbp-2; VDUP1; Hyplip1; AA682105; 1200008J08Rik
NCBI Protein Information
thioredoxin-interacting protein
UniProt Protein Name
Thioredoxin-interacting protein
UniProt Gene Name
Txnip
UniProt Synonym Gene Names
Vdup1
UniProt Entry Name
TXNIP_MOUSE
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Product Notes
The Mouse TXNIP txnip (Catalog #AAA31514) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA31514 ELISA Kit recognizes Mouse TXNIP. It is sometimes possible for the material contained within the vial of "Thioredoxin-Interacting Protein (TXNIP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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