Standard Curve (Sample)
Mouse Ghrelin O-Acyltransferase (MBOAT4) ELISA Kit | GOAT elisa kit
Mouse Ghrelin O-Acyltransferase (MBOAT4) ELISA Kit
Gene Names
Mboat4; GOAT; Gm171
Reactivity
Mouse
Synonyms
Ghrelin O-Acyltransferase (MBOAT4); Mouse Ghrelin O-Acyltransferase (MBOAT4) ELISA Kit; MBOAT4; FKSG89; OACT4; O-acyltransferase (membrane bound) domain containing 4; ghrelin O-acyltransferase; GOAT elisa kit
Reactivity
Mouse
Sequence Length
435
Assay Type
Sandwich
Samples
Mouse serum, cell culture supernates and other biological fluids
Detection Range
10 pg/ml - 160 pg/ml
Sensitivity
< 1 pg/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GOAT elisa kit
Background: GOAT could modify ghrelin ser3 with fatty acids up to tetradecanoic acid. Replacement of his338 of GOAT with ala completely abolished the ability of GOAT to octanoylate ghrelin.mouse Goat octanoylated ghrelin (GHRL), a 28-amino acid appetite-stimulating peptide hormone, following cotransfection of Goat and preproghrelin in cultured endocrine cell lines.
Principle of the Assay: Mouse Ghrelin O-acyltransferase (MBOAT4) ELISA Kit employs a two-site sandwich ELISA to quantitate MBOAT4 in samples. An antibody specific for MBOAT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBOAT4 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Mouse MBOAT4 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MBOAT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Mouse Ghrelin O-acyltransferase (MBOAT4) ELISA Kit employs a two-site sandwich ELISA to quantitate MBOAT4 in samples. An antibody specific for MBOAT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBOAT4 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Mouse MBOAT4 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MBOAT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ghrelin O-acyltransferase
NCBI Official Synonym Full Names
membrane bound O-acyltransferase domain containing 4
NCBI Official Symbol
Mboat4
NCBI Official Synonym Symbols
GOAT; Gm171
NCBI Protein Information
ghrelin O-acyltransferase
UniProt Protein Name
Ghrelin O-acyltransferase
UniProt Gene Name
Mboat4
UniProt Synonym Gene Names
Gm171; Goat; O-acyltransferase domain-containing protein 4
UniProt Entry Name
MBOA4_MOUSE
Similar Products
Product Notes
The Mouse GOAT mboat4 (Catalog #AAA31515) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA31515 ELISA Kit recognizes Mouse GOAT. It is sometimes possible for the material contained within the vial of "Ghrelin O-Acyltransferase (MBOAT4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
