Mouse CD90 Monoclonal Antibody | anti-CD90 antibody
MOUSE ANTI RAT CD90:Low Endotoxin
Gene Names
Thy1; CD7
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
CD90, Antibody; MOUSE ANTI RAT CD90:Low Endotoxin; anti-CD90 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-7
Form/Format
Low Endotoxin
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
161
Applicable Applications for anti-CD90 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/40; Maximum Dilution: 1/80
Flow Cytometry: Minimum Dilution: 1/40; Maximum Dilution: 1/80
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Rat
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-CD90 antibody
Mouse anti Rat CD90 antibody, clone OX-7 recognizes rat and CD90, also known as Thy1.1, a GPI-anchored membrane protein containing a single V type Ig-like domain CD90 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, immature B cells and connective tissues, CD90 is also expressed in T cells in mice.Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab' of MRC OX-7 for rat Thy1 is 3 x 109m1 and for mouse Thy1.1 is 3 x 108m-1(1). Mouse anti rat CD90, clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (Jeng et al. 1998). Clone OX-7 has also been reported to induce glomerular nephritis in Wistar rats (Tamura et al. 1996). This product is routinely tested in flow cytometry on rat thymocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,172 Da
NCBI Official Full Name
thy-1 membrane glycoprotein preproprotein
NCBI Official Synonym Full Names
Thy-1 cell surface antigen
NCBI Official Symbol
Thy1
NCBI Official Synonym Symbols
CD7
NCBI Protein Information
thy-1 membrane glycoprotein; CD90; Thy-1 protein; Thymus cell surface antigen; thy-1 antigen; thy-1 glycoprotein; thymus cell antigen 1, theta
UniProt Protein Name
Thy-1 membrane glycoprotein
UniProt Gene Name
Thy1
UniProt Synonym Gene Names
Thy-1
UniProt Entry Name
THY1_RAT
