Purity
(The purity of RIPK1 was determined to be >70% by densitometry, approx. MW 108 kDa.)
RIPK1 active protein
RIPK1, Active
Purity
>70%
Synonyms
RIPK1; N/A; RIPK1, Active; RIPK1 active protein
Purity/Purification
>70%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.05ug/ul (varies by lot)
Gene Aliases
RCK; MAP3K19
Specific Activity
1.4 nmol/ min/mg
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Ships on dry ice. Store product at -70°C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity
(The purity of RIPK1 was determined to be >70% by densitometry, approx. MW 108 kDa.)
Purity
(The purity of RIPK1 was determined to be >70% by densitometry, approx. MW 108 kDa.)
Specific Activity
(The specific activity of RIPK1 was determined to be 1.4 nmol/min/mg as per activity assay protocol, and was equivalent to 3 nmol/min/mg as per radiometric assay.)
Specific Activity
(The specific activity of RIPK1 was determined to be 1.4 nmol/min/mg as per activity assay protocol, and was equivalent to 3 nmol/min/mg as per radiometric assay.)
Related Product Information for RIPK1 active protein
Description: Full-length recombinant human RIPK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background : RIPK1 or Receptor Interacting Protein Kinase 1 is a serine/threonine kinase that was originally identified as interacting with the cytoplasmic domain of FAS. RIPK1 has been deemed as an important element in the signal transduction machinery that mediates programmed cell death. RIPK1 has been shown to interact with TRADD, TRAF1 TRAF2 and TRAF3 and TRADD can act as an adaptor protein to recruit RIPK1to the TNFR1 complex in a TNF-dependent process (1). TNFα is capable of activating the noncanonical NF-κB pathway, but this activation of this pathway is negatively regulated by RIPK1(2).
Scientific Background : RIPK1 or Receptor Interacting Protein Kinase 1 is a serine/threonine kinase that was originally identified as interacting with the cytoplasmic domain of FAS. RIPK1 has been deemed as an important element in the signal transduction machinery that mediates programmed cell death. RIPK1 has been shown to interact with TRADD, TRAF1 TRAF2 and TRAF3 and TRADD can act as an adaptor protein to recruit RIPK1to the TNFR1 complex in a TNF-dependent process (1). TNFα is capable of activating the noncanonical NF-κB pathway, but this activation of this pathway is negatively regulated by RIPK1(2).
NCBI and Uniprot Product Information
Similar Products
Product Notes
The RIPK1 (Catalog #AAA14253) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RIPK1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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