Human Agrin ELISA Kit | AGRN elisa kit
Human Agrin ELISA Kit
Gene Names
AGRN; CMS8; CMSPPD
Reactivity
Human
Synonyms
Agrin; N/A; Human Agrin ELISA Kit; AGRN; AGRIN; AGRN elisa kit
Reactivity
Human
Specificity
Recombinant and natural Human Agrin
Sequence Length
2045
Samples
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Related Product Information for AGRN elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
216,733 Da
NCBI Official Full Name
agrin isoform 2
NCBI Official Synonym Full Names
agrin
NCBI Official Symbol
AGRN
NCBI Official Synonym Symbols
CMS8; CMSPPD
NCBI Protein Information
agrin
UniProt Protein Name
Agrin
UniProt Gene Name
AGRN
UniProt Synonym Gene Names
AGRIN; C90; C22
UniProt Entry Name
AGRIN_HUMAN
