Standard Curve (Sample)
Human Alpha 1 Microglobulin ELISA Kit | Alpha 1 MG elisa kit
Human Alpha 1 Microglobulin ELISA Kit
Gene Names
AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
Reactivity
Human
Synonyms
Alpha 1 Microglobulin; N/A; Human Alpha 1 Microglobulin ELISA Kit; Alpha 1 MG elisa kit
Reactivity
Human
Samples
Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Alpha 1 MG elisa kit
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Intended Uses: This A1-MG ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of A1-MG in the sample, this A1-MG ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus A1-MG concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of A1-MG utilizes a multiclonal anti-A1-MG antibody and an A1-MG-HRP conjugate. The assay sample and buffer are incubated together with A1-MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A1-MG concentration since A1-MG from samples and A1-MG-HRP conjugate compete for the anti-A1-MG antibody binding site. Since the number of sites is limited, as more sites are occupied by A1-MG from the sample, fewer sites are left to bind A1-MG-HRP conjugate. Standards of known A1-MG concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of A1-MG. The A1-MG concentration in each sample is interpolated from this standard curve.
Intended Uses: This A1-MG ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of A1-MG in the sample, this A1-MG ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus A1-MG concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of A1-MG utilizes a multiclonal anti-A1-MG antibody and an A1-MG-HRP conjugate. The assay sample and buffer are incubated together with A1-MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A1-MG concentration since A1-MG from samples and A1-MG-HRP conjugate compete for the anti-A1-MG antibody binding site. Since the number of sites is limited, as more sites are occupied by A1-MG from the sample, fewer sites are left to bind A1-MG-HRP conjugate. Standards of known A1-MG concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of A1-MG. The A1-MG concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Alpha 1 MG elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
38,999 Da
NCBI Official Full Name
alpha-1-microglobulin, partial
NCBI Official Synonym Full Names
alpha-1-microglobulin/bikunin precursor
NCBI Official Symbol
AMBP
NCBI Official Synonym Symbols
A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
NCBI Protein Information
protein AMBP; bikunin; uristatin; protein HC; trypstatin; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge
UniProt Protein Name
Protein AMBP
UniProt Gene Name
AMBP
UniProt Synonym Gene Names
HCP; ITIL; Protein HC; ITI-LC
UniProt Entry Name
AMBP_HUMAN
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Product Notes
The Human Alpha 1 MG ambp (Catalog #AAA83271) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA83271 ELISA Kit recognizes Human Alpha 1 MG. It is sometimes possible for the material contained within the vial of "Alpha 1 Microglobulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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