Human Apolipoprotein B48 ELISA Kit | Apo B48 elisa kit

Human Apolipoprotein B48 ELISA Kit

Cat. Num. AAA96125

• Gene Names: APOB; FLDB; LDLCQ4
• Reactivity: Human
• Synonyms: Apolipoprotein B48; N/A; Human Apolipoprotein B48 ELISA Kit; Apo B48 elisa kit

• Reactivity: Human
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250- 5000ng/mL
• Sensitivity: 1.0ng/mL

• Intended Uses: This Apo-B48 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Apo-B48. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for Apo B48 elisa kit

Principle of the assay: Apo-B48 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Apo-B48 antibody and an Apo-B48-HRP conjugate. The assay sample and buffer are incubated together with Apo-B48-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Apo-B48 concentration since Apo-B48 from samples and Apo-B48-HRP conjugate compete for the anti-Apo-B48 antibody binding site. Since the number of sites is limited, as more sites are occupied by Apo-B48 from the sample, fewer sites are left to bind Apo-B48-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Apo-B48 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Apo B48 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 178802
• NCBI GeneID: 338
• Molecular Weight: 515,605 Da
• NCBI Official Full Name: apolipoprotein B-48, partial
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: APOB
• NCBI Official Synonym Symbols: FLDB; LDLCQ4
• NCBI Protein Information: apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
• UniProt Protein Name: Apolipoprotein B-100
• UniProt Gene Name: APOB
• UniProt Synonym Gene Names: Apo B-100; Apo B-48
• UniProt Entry Name: APOB_HUMAN