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product-image-AAA112874_SC15.png Standard Curve (Sample)

Bovine apolipoprotein A-I ELISA Kit | APOA1 elisa kit

Bovine Apolipoprotein A-I, APOA1 ELISA Kit

Reactivity
Bovine
Synonyms
apolipoprotein A-I; N/A; Bovine Apolipoprotein A-I, APOA1 ELISA Kit; Bovine Apolipoprotein A-I (APOA1) ELISA kit; MGC117399; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348; APOA1 elisa kit
Ordering
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of Bovine APOA1. No significant cross-reactivity or interference between Bovine APOA1 and analogues was observed.
Sequence Length
265
Samples
Serum, plasma, tissue homogenates
Assay Type
Competitive
Detection Range
78 ng/ml-5000 ng/ml
Sensitivity
19.5 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
Lipogenesis and lipometabolism
Protein Biological Process 3
Cholesterol metabolism
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

product-image-AAA112874_SC15.png Standard Curve (Sample)
Related Product Information for APOA1 elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with APOA1. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for APOA1. The competitive inhibition reaction is launched between with pre-coated APOA1 and APOA1 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of APOA1 in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,276 Da
NCBI Official Full Name
apolipoprotein A-I preproprotein
NCBI Official Synonym Full Names
apolipoprotein A-I
NCBI Official Symbol
APOA1
NCBI Protein Information
apolipoprotein A-I; apo-AI; apoA-I; apolipoprotein A1; apolipoprotein A-I preproprotein
UniProt Protein Name
Apolipoprotein A-I
UniProt Gene Name
APOA1
UniProt Synonym Gene Names
Apo-AI; ApoA-I; ProapoA-I
UniProt Entry Name
APOA1_BOVIN

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Product Notes

The Bovine APOA1 apoa1 (Catalog #AAA112874) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA112874 ELISA Kit recognizes Bovine APOA1. It is sometimes possible for the material contained within the vial of "apolipoprotein A-I, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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