Application Data
CytoSelect 24-well Cell Migration Assay (8 um), Fluorometric Assay Kit
CytoSelect 24-well Cell Migration Assay (8 um), Fluorometric
Synonyms
CytoSelect 24-well Cell Migration Assay (8 um), Fluorometric; N/A; CytoSelect 24-well Cell Migration Assay (8 um), Fluorometric assay kit
Preparation and Storage
Store all components at 4 degree C until their expiration dates.
Application Data
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Related Product Information for CytoSelect 24-well Cell Migration Assay (8 um), Fluorometric assay kit
Background: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors). CytoSelect Cell Migration Assay Kit utilizes polycarbonate membrane inserts (8 mum pore size) to assay the migratory properties of cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-migratory cells (i.e. cotton swabbing). Any migratory cells are first dissociated from the membrane, then lysed and detected by the patented CyQuant GR Dye (Invitrogen). CytoSelect Cell Migration Assay Kit provides a robust system for the quantitative determination of cell migration. The kit contains sufficient reagents for the evaluation of 12 samples. The 8 mum pore size is optimal for epithelial and fibroblast cell migration. However, in the case of leukocyte chemotaxis, a smaller pore size (3 mum) is recommended. The CytoSelect Cell Migration Assay Kit contains polycarbonate membrane inserts (8 mum pore size) in a 24-well plate. The membrane serves as a barrier to discriminate migratory cells from non-migratory cells. Migratory cells are able to extend protrusions towards chemoattractants (via actin cytoskeleton reorganization) and ultimately pass through the pores of the polycarbonate membrane. These migratory cells are then dissociated from the membrane and subsequently detected by the patented CyQuant GR Dye (Invitrogen).
Product Categories/Family for CytoSelect 24-well Cell Migration Assay (8 um), Fluorometric assay kit
References
1. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT, Horwitz AR. (2003) Science 302:1704-9.
2. Horwitz R, Webb D. (2003) Curr Biol. 13:R756-9.
3. Lauffenburger DA, Horwitz AF. (1996) Cell 84:359-369.
1. Izhak, L. et al. (2010). Predominant expression of CCL2 at the tumor site of prostate cancer patients directs a selective loss of immunological tolerance to CCL2 that could be amplified in a beneficial manner. J. Immunol. 184:1092-1101.
2. Wang, W. et al. (2009). Netrin-1 increases proliferation and migration of renal proximal tubular epithelial cells via the UNC5B receptor. Am. J. Physiol. Renal Physiol. 296:F723-729.3. Izhak, L. et al. (2009). A novel recombinant fusion protein encoding a 20-amino acid residue of the third extracellular (E3) domain of CCR2 neutralizes the biological acitivity of CCL2. J. Immunol. 183:732-739.
4. Zhao, L. et al. (2008). CD44 expressed on both bone marrow-derived and non-bone marrow-derived cells promotes atherogenesis in ApoE-deficient mice. Arterioscler. Thromb. Vasc. Biol. 28:1283-1289.
5. Fava, G. et al. (2008). Leptin enhances cholangiocarcinoma cell growth. Cancer Res. 68:6752-6761.
6. Uddin, M. et al. (2008). Marinobufagenin inhibits proliferation and migration of cytotrophoblast and CHO cells. Placenta 29(3):266-273.
7. Reing, J. et al. (2008). Degradation products of extracellular matrix affect cell migration and proliferation. Tissue Eng. Part A 10.1089/ten.tea.2007.0425.
2. Horwitz R, Webb D. (2003) Curr Biol. 13:R756-9.
3. Lauffenburger DA, Horwitz AF. (1996) Cell 84:359-369.
1. Izhak, L. et al. (2010). Predominant expression of CCL2 at the tumor site of prostate cancer patients directs a selective loss of immunological tolerance to CCL2 that could be amplified in a beneficial manner. J. Immunol. 184:1092-1101.
2. Wang, W. et al. (2009). Netrin-1 increases proliferation and migration of renal proximal tubular epithelial cells via the UNC5B receptor. Am. J. Physiol. Renal Physiol. 296:F723-729.3. Izhak, L. et al. (2009). A novel recombinant fusion protein encoding a 20-amino acid residue of the third extracellular (E3) domain of CCR2 neutralizes the biological acitivity of CCL2. J. Immunol. 183:732-739.
4. Zhao, L. et al. (2008). CD44 expressed on both bone marrow-derived and non-bone marrow-derived cells promotes atherogenesis in ApoE-deficient mice. Arterioscler. Thromb. Vasc. Biol. 28:1283-1289.
5. Fava, G. et al. (2008). Leptin enhances cholangiocarcinoma cell growth. Cancer Res. 68:6752-6761.
6. Uddin, M. et al. (2008). Marinobufagenin inhibits proliferation and migration of cytotrophoblast and CHO cells. Placenta 29(3):266-273.
7. Reing, J. et al. (2008). Degradation products of extracellular matrix affect cell migration and proliferation. Tissue Eng. Part A 10.1089/ten.tea.2007.0425.
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Product Notes
The CytoSelect 24-well Cell Migration Assay (8 um), Fluorometric (Catalog #AAA44547) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CytoSelect 24-well Cell Migration Assay (8 um), Fluorometric, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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