Standard Curve (Sample)
CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric) Assay Kit
CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric)
Synonyms
CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric); N/A; CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric) assay kit
Preparation and Storage
Store all kit components at 4 degree C up to their expiration dates.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric) assay kit
Principle of the Assay: The CytoSelect™ Cell Adhesion Assay Kit utilizes an ECM protein-coated 48-well plate (see Adhesion Plate Layout below). First, cells are seeded onto the coated substrate, where the adherent cells are captured. Next, unbound cells are washed away, and the adherent cells are fixed/stained. Finally, the stain is extracted and quantified colorimetrically.
Background/Introduction: Cell adhesion is a complex process involved in embryogenesis, migration/invasion, tissue remodeling, and wound healing. To perform these processes, cells adhere to extracellular matrix components (via adhesion receptors), forming complexes with components of the cytoskeleton that ultimately affect cell motility, differentiation, proliferation, and survival. The CytoSelect™ Cell Adhesion Assay Kit provides a rapid, quantitative method for evaluating cell adhesion. The kit contains sufficient reagents for the evaluation of 48 samples (40 ECM protein-coated wells, 8 BSA-coated wells).
Background/Introduction: Cell adhesion is a complex process involved in embryogenesis, migration/invasion, tissue remodeling, and wound healing. To perform these processes, cells adhere to extracellular matrix components (via adhesion receptors), forming complexes with components of the cytoskeleton that ultimately affect cell motility, differentiation, proliferation, and survival. The CytoSelect™ Cell Adhesion Assay Kit provides a rapid, quantitative method for evaluating cell adhesion. The kit contains sufficient reagents for the evaluation of 48 samples (40 ECM protein-coated wells, 8 BSA-coated wells).
Product Categories/Family for CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric) assay kit
References
1. Hynes, R. O. (1992) Cell 69:11-25.
2. Schwartz, M. A., Schaller, M. D. and Ginsberg, M. H. (1995) Annu. Rev. Cell Dev. Biol. 11:549-599.
1. Tanoury, Z, et al. (2014). Genes involved in cell adhesion and signaling: a new repertoire of retinoic acid receptor target genes in mouse embryonic fibroblasts. J. Cell Sci. 127:521-533.
2. Xing, C. et al. (2013). Reversing effect of ring finger protein 43 inhibition on malignant phenotypes of human hepatocellular carcinoma. Mol. Cancer Ther. 12:94-103.
3. Mei, J. et al. (2012). Inhibition of IDO1 suppresses cyclooxygenase-2 and matrix metalloproteinase-9 expression and decreases proliferation, adhesion and invasion of endometrial stromal cells. Mol. Human. Reprod. 10.1093/molehr/gas021.
4. Kandalam, V. et al. (2011). Lack of tissue inhibitor of metalloproteinases 2 leads to exacerbated left ventricular dysfunction and adverse extracellular matrix remodeling in response to biomechanical stress. Circulation 124:2094-2105.
5. Tsukamoto, H. et al. (2011). Evaluation of anticancer activities of benzo[c]phenanthridine alkaloid sanguinarine in oral squamous cell carcinoma cell line. Anticancer Res. 31:2841-2846.
6. Cervera, A.M. et al. (2008). Cells silenced for SDHB expression display characteristic features of the tumor phenotype. Cancer Res. 68:4058-4067.
2. Schwartz, M. A., Schaller, M. D. and Ginsberg, M. H. (1995) Annu. Rev. Cell Dev. Biol. 11:549-599.
1. Tanoury, Z, et al. (2014). Genes involved in cell adhesion and signaling: a new repertoire of retinoic acid receptor target genes in mouse embryonic fibroblasts. J. Cell Sci. 127:521-533.
2. Xing, C. et al. (2013). Reversing effect of ring finger protein 43 inhibition on malignant phenotypes of human hepatocellular carcinoma. Mol. Cancer Ther. 12:94-103.
3. Mei, J. et al. (2012). Inhibition of IDO1 suppresses cyclooxygenase-2 and matrix metalloproteinase-9 expression and decreases proliferation, adhesion and invasion of endometrial stromal cells. Mol. Human. Reprod. 10.1093/molehr/gas021.
4. Kandalam, V. et al. (2011). Lack of tissue inhibitor of metalloproteinases 2 leads to exacerbated left ventricular dysfunction and adverse extracellular matrix remodeling in response to biomechanical stress. Circulation 124:2094-2105.
5. Tsukamoto, H. et al. (2011). Evaluation of anticancer activities of benzo[c]phenanthridine alkaloid sanguinarine in oral squamous cell carcinoma cell line. Anticancer Res. 31:2841-2846.
6. Cervera, A.M. et al. (2008). Cells silenced for SDHB expression display characteristic features of the tumor phenotype. Cancer Res. 68:4058-4067.
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Product Notes
The CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric) (Catalog #AAA11428) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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