Application Data
Ferroptosis Inhibitory Protein-1 (FSP-1) Assay Kit | FSP-1 assay kit
Ferroptosis Inhibitory Protein-1 (FSP-1) Activity Fluorometric Assay Kit
Synonyms
Ferroptosis Inhibitory Protein-1 (FSP-1); N/A; Ferroptosis Inhibitory Protein-1 (FSP-1) Activity Fluorometric Assay Kit; FSP-1 assay kit
Detection Type
Fluorescence
Result Type
Enzyme activity
Samples
Animal and plant tissue, Cell
Detection Range
0.15-5.00U/L
Sensitivity
0.15U/L
Precision
Intra-assay CV: 3.0-5.4%
Inter-assay CV: 6.5-8.0%
Inter-assay CV: 6.5-8.0%
Detection Instrument
Fluorescence microplate reader
Preparation and Storage
This product can be stored at -20 degree C for 12 months with shading light.
Application Data
Application Data
Application Data
Application Data
Related Product Information for FSP-1 assay kit
Ferroptosis inhibitory protein-1(FSP-1) induce non-caspase-dependent apoptosis based on factors such as C-terminal fragment of amino acid series, nuclear translocation and overexpression. Through the FSP1-CoQ10-NAD(P)H pathway and the classical glutathione (GSH)-GPX4 pathway, FSP-1 can protect cells from ferroptosis, and plays a "double-edged sword" role in cell life activities.
The detection principle of this kit: FSP-1 catalyzes the substrate reaction to generate NADH, so that its fluorescence value increases under the excitation wavelength of 535nm and emission wavelength of 587nm. Adding inhibitors will inhibit the activity of FSP-1 and cause the rate of increase in fluorescence value to decrease, and the activity of FSP-1 can be calculated by measuring the difference value.
The detection principle of this kit: FSP-1 catalyzes the substrate reaction to generate NADH, so that its fluorescence value increases under the excitation wavelength of 535nm and emission wavelength of 587nm. Adding inhibitors will inhibit the activity of FSP-1 and cause the rate of increase in fluorescence value to decrease, and the activity of FSP-1 can be calculated by measuring the difference value.
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Product Notes
The FSP-1 (Catalog #AAA179684) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ferroptosis Inhibitory Protein-1 (FSP-1), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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