Luciferase Assay Kit
Firefly Luciferase Assay Kit
Synonyms
Luciferase; N/A; Firefly Luciferase Assay Kit; Luciferase assay kit
Application
For firefly luciferase reporter assay
Features
- Greater linear range
- Accurate
- Robust: Excellent signal to noise (basal) ratio. Stable assay signal.
- Easy to use: Homogenous assay, add-and-read assay, amenable to HTS format.
- Accurate
- Robust: Excellent signal to noise (basal) ratio. Stable assay signal.
- Easy to use: Homogenous assay, add-and-read assay, amenable to HTS format.
Kit Components
Note: Provided in the sizes of 10 mL (for one plate) and 100 mL (for 10 plates)
For 10 ml (for one plate):
- Solution I and II: 5 ml each
- Component A: 35 ul
- Component B: 120 ul
For 100 ml (for 10 plates):
- Solution I and II: 50 ml each
- Component A: 350 ul
- Component B: 1.2 ml
For 10 ml (for one plate):
- Solution I and II: 5 ml each
- Component A: 35 ul
- Component B: 120 ul
For 100 ml (for 10 plates):
- Solution I and II: 50 ml each
- Component A: 350 ul
- Component B: 1.2 ml
Application Notes
Assay Protocol (for one 96-well or 384-well plate)
A) Preparation of Luciferase Assay WORKING SOLUTION
1. Thaw Solution I and Solution II at room temperature (RT, ~22°C).
2. Thaw components A & B at RT and then keep them on ice. Note: Do not thaw Component A& B at 37°C.
3. Prepare Assay Buffer by mixing Solution I (5 ml) and Solution II (5 ml). Transfer Component A (35 ul) and Component B (120 ul) into the 10 ml of Assay Buffer to make luciferase assay WORKING SOLUTION. The amount of WORKING SOLUTION prepared can be modified to suit the experimental design. Note: Unused Components A and B can be refrozen (at -20°C). Unused Assay Buffer can be stored at 4°C to be used for later experiments the same day.
4. Mix thoroughly by inverting and vortexing for 30 sec. The reconstituted WORKING SOLUTION at RT should be used within 1 hour, or it can be kept at 4°C for no more than 4 hours. In general, only the freshly prepared WORKING SOLUTION is recommended for the experiments.
B) Measurement of Luciferase Activity in Growing Cells
1. Grow cells on opaque white 96 well plates (please inquire) appropriate for luciferase signal measurement.
2. To generate assay signal, add an equal volume of luciferase assay WORKING SOLUTION to culture media overlaying cells.
3. Incubate the plate at room temperature (~22°C) for 5 minutes.
4. Read on a luminescence plate reader and record the data.
A) Preparation of Luciferase Assay WORKING SOLUTION
1. Thaw Solution I and Solution II at room temperature (RT, ~22°C).
2. Thaw components A & B at RT and then keep them on ice. Note: Do not thaw Component A& B at 37°C.
3. Prepare Assay Buffer by mixing Solution I (5 ml) and Solution II (5 ml). Transfer Component A (35 ul) and Component B (120 ul) into the 10 ml of Assay Buffer to make luciferase assay WORKING SOLUTION. The amount of WORKING SOLUTION prepared can be modified to suit the experimental design. Note: Unused Components A and B can be refrozen (at -20°C). Unused Assay Buffer can be stored at 4°C to be used for later experiments the same day.
4. Mix thoroughly by inverting and vortexing for 30 sec. The reconstituted WORKING SOLUTION at RT should be used within 1 hour, or it can be kept at 4°C for no more than 4 hours. In general, only the freshly prepared WORKING SOLUTION is recommended for the experiments.
B) Measurement of Luciferase Activity in Growing Cells
1. Grow cells on opaque white 96 well plates (please inquire) appropriate for luciferase signal measurement.
2. To generate assay signal, add an equal volume of luciferase assay WORKING SOLUTION to culture media overlaying cells.
3. Incubate the plate at room temperature (~22°C) for 5 minutes.
4. Read on a luminescence plate reader and record the data.
Preparation and Storage
Store all in freezer (-20°C).
Similar Products
Product Notes
The Luciferase (Catalog #AAA13785) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Assay Protocol (for one 96-well or 384-well plate) A) Preparation of Luciferase Assay WORKING SOLUTION 1. Thaw Solution I and Solution II at room temperature (RT, ~22°C). 2. Thaw components A & B at RT and then keep them on ice. Note: Do not thaw Component A& B at 37°C. 3. Prepare Assay Buffer by mixing Solution I (5 ml) and Solution II (5 ml). Transfer Component A (35 ul) and Component B (120 ul) into the 10 ml of Assay Buffer to make luciferase assay WORKING SOLUTION. The amount of WORKING SOLUTION prepared can be modified to suit the experimental design. Note: Unused Components An and B can be refrozen (at -20°C). Unused Assay Buffer can be stored at 4°C to be used for later experiments the same day. 4. Mix thoroughly by inverting and vortexing for 30 sec. The reconstituted WORKING SOLUTION at RT should be used within 1 hour, or it can be kept at 4°C for no more than 4 hours. In general, only the freshly prepared WORKING SOLUTION is recommended for the experiments. B) Measurement of Luciferase Activity in Growing Cells 1. Grow cells on opaque white 96 well plates (please inquire) appropriate for luciferase signal measurement. 2. To generate assay signal, add an equal volume of luciferase assay WORKING SOLUTION to culture media overlaying cells. 3. Incubate the plate at room temperature (~22°C) for 5 minutes. 4. Read on a luminescence plate reader and record the data. Researchers should empirically determine the suitability of the Luciferase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Luciferase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.