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Application Data
Rac1/Cdc42 Activation Assay Kit
Rac1/Cdc42 Activation Assay Combo Kit
Synonyms
Rac1/Cdc42 Activation; N/A; Rac1/Cdc42 Activation Assay Combo Kit; Rac1/Cdc42 Activation assay kit
Preparation and Storage
Store all kit components at -20 degree C until their expiration dates. Avoid multiple freeze/thaw cycles.
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Related Product Information for Rac1/Cdc42 Activation assay kit
Background/Introduction: Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Rac1 and Cdc42, 21 kDa proteins, belong to the family of Rho GTPases regulates a variety of biological response pathways that include cell motility, cell division, gene transcription, and cell transformation. Like other small GTPases, Rac1 and Cdc42 regulate molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rac1 or Cdc42 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades. Rac1/Cdc42 Activation Assay utilizes PAK PBD Agarose beads to selectively isolate and pull-down the active form of Rac or Cdc42 from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rac or Cdc42 is detected by western blot analysis using an anti-Rac1 or Cdc42 specific monoclonal antibody (see Figure 3, Figure 5 and Assay Principle). Rac1/Cdc42 Activation Assay Kit provides a simple and fast tool to monitor the activation of Rac1 or Cdc42. The kit includes easily identifiable PAK1 PBD Agarose beads (see Figure 1), pink in color, along with Rac1 and Cdc42 Immunoblot Positive Controls for quick Rac1 or Cdc42 identification. Each kit provides sufficient quantities to perform 20 assays for each small GTPase.
Product Categories/Family for Rac1/Cdc42 Activation assay kit
References
1. Raftopoulou M., and Hall A. (2004) Dev Biol. 265: 23-32.
2. Bar-Sagi D., and Hall A. (2000) Cell 103: 227-38.
3. Benard, V., Bohl, B. P., and Bokoch, G. M. (1999) J. Biol. Chem. 274, 13198-13204.
1. Chen, H. et al. (2010). Integrity of SOS1/EPS8/ABI1 Tri-Complex Determines Ovarian Cancer Metastasis. Cancer Res. 70:9979-9990.
2. Sultana, H. et al. (2010). Anaplasma Phagocytophilum Induces Actin Phosphorylation to Selectively Regulate Gene Transcription in Ixodes Scapularis Ticks. J. Exp. Med. 10.1084/jem.20100276.
3. Pandey, D. et al. (2009). Unraveling a Novel Rac1-Mediated Signaling Pathway that Regulates Cofilin Dephosphorylation and Secretion in Thrombin Stimulated Platelets. Blood 114:415-424.
4. Lorger, M. et al. (2006). Regulation of Epithelial Wound Closure and Intercellular Adhesion by Interaction of AF6 with Actin Cytoskeleton. J. of Cell Science 119:3385-3398.
2. Bar-Sagi D., and Hall A. (2000) Cell 103: 227-38.
3. Benard, V., Bohl, B. P., and Bokoch, G. M. (1999) J. Biol. Chem. 274, 13198-13204.
1. Chen, H. et al. (2010). Integrity of SOS1/EPS8/ABI1 Tri-Complex Determines Ovarian Cancer Metastasis. Cancer Res. 70:9979-9990.
2. Sultana, H. et al. (2010). Anaplasma Phagocytophilum Induces Actin Phosphorylation to Selectively Regulate Gene Transcription in Ixodes Scapularis Ticks. J. Exp. Med. 10.1084/jem.20100276.
3. Pandey, D. et al. (2009). Unraveling a Novel Rac1-Mediated Signaling Pathway that Regulates Cofilin Dephosphorylation and Secretion in Thrombin Stimulated Platelets. Blood 114:415-424.
4. Lorger, M. et al. (2006). Regulation of Epithelial Wound Closure and Intercellular Adhesion by Interaction of AF6 with Actin Cytoskeleton. J. of Cell Science 119:3385-3398.
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Product Notes
The Rac1/Cdc42 Activation (Catalog #AAA44550) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Rac1/Cdc42 Activation, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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