Rabbit CDC42 Antibody | anti-CDC42 antibody

CDC42 Polyclonal Antibody

Cat. Num. AAA36831

• Gene Names: CDC42; G25K; CDC42Hs
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity purification
• Synonyms: CDC42; N/A; CDC42 Polyclonal Antibody; CDC42Hs; G25K; TKS; anti-CDC42 antibody

• Host: Rabbit
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.

• Applicable Applications for anti-CDC42 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Species Reactivity/Cross-Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-191 of human CDC42 (NP_001782.1).
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH-3T3 cells using CDC42 Rabbit pAb (AAA36831) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of C6 cells using CDC42 Rabbit pAb (AAA36831) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded rat testis using CDC42 antibody (AAA36831) at dilution of 1:100 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using CDC42 antibody (AAA36831) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Detection: ECL Basic Kit (RM00020).)

Related Product Information for anti-CDC42 antibody

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20.

Product Categories/Family for anti-CDC42 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 322510015
• NCBI GeneID: 998
• UniProt Accession #: P60953; P21181; P25763; Q7L8R5; Q9UDI2
• Molecular Weight: Observed MW: 21KDa; Calculated MW: 21kDa
• NCBI Official Full Name: Cell division control protein 42 homolog
• NCBI Official Synonym Full Names: cell division cycle 42
• NCBI Official Symbol: CDC42
• NCBI Official Synonym Symbols: G25K; CDC42Hs
• NCBI Protein Information: cell division control protein 42 homolog; G25K GTP-binding protein; GTP-binding protein, 25kD; growth-regulating protein; GTP binding protein, 25kDa; small GTP binding protein CDC42; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))
• UniProt Protein Name: Cell division control protein 42 homolog
• UniProt Gene Name: CDC42
• UniProt Entry Name: CDC42_HUMAN