Application Data
CDC42 recombinant protein
CDC42, Active
Gene Names
CDC42; TKS; G25K; CDC42Hs
Applications
Western Blot, ELISA
Synonyms
CDC42; N/A; CDC42, Active; CDC42 recombinant protein
Host
E Coli
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
Full length
Sequence Length
2182
Applicable Applications for CDC42 recombinant protein
WB (Western Blot), ELISA
Species
Human
Tag
GST/HIS
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for CDC42 recombinant protein
Description: Recombinant full length human CDC42 was expressed in E Coli cells using an N-dual GST/His tag.
Scientific Background: CDC42 is a member of the Rho family of GTPases and is part of cellular pathways fundamental to growth, differentiation and apoptosis (1). Downstream targets for CDC42 include those that regulate the actin cytoskeleton (e.g. WASP) and cellular stress pathways (e.g. PAK) as well as the coatomer protein complex and PAR6. CDC42 is involved in the G (1)-S progression of the cell cycle. CDC42 and its downstream effector mDia3 are involved in bi-orientation and stabilization of spindle microtubules attachment to kinetochores and regulate chromosome alignment and segregation during mitosis (2).
Scientific Background: CDC42 is a member of the Rho family of GTPases and is part of cellular pathways fundamental to growth, differentiation and apoptosis (1). Downstream targets for CDC42 include those that regulate the actin cytoskeleton (e.g. WASP) and cellular stress pathways (e.g. PAK) as well as the coatomer protein complex and PAR6. CDC42 is involved in the G (1)-S progression of the cell cycle. CDC42 and its downstream effector mDia3 are involved in bi-orientation and stabilization of spindle microtubules attachment to kinetochores and regulate chromosome alignment and segregation during mitosis (2).
Product Categories/Family for CDC42 recombinant protein
References
1. Erickson, J.W. et al: Multiple roles for Cdc42 in cell regulation. Curr Opin Cell Biol. 2001 Apr;13 (2):153-7. 2. Narumyia, S. et al: A new look at Rho GTPases in cell cycle: role in kinetochore-microtubule attachment. Cell Cycle. 2004 Jul;3 (7):855-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~48 kDa
NCBI Official Full Name
Homo sapiens cell division cycle 42 (CDC42), transcript variant 1, mRNA
NCBI Official Synonym Full Names
cell division cycle 42
NCBI Official Symbol
CDC42
NCBI Official Synonym Symbols
TKS; G25K; CDC42Hs
NCBI Protein Information
cell division control protein 42 homolog
UniProt Protein Name
Cell division control protein 42 homolog
UniProt Gene Name
CDC42
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Product Notes
The CDC42 cdc42 (Catalog #AAA72959) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full length. AAA Biotech's CDC42 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CDC42 cdc42 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC42, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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