Bioactivity
(Human CD47 is coated at 1 ug/ml (100 ul/well) can bind Human SIRP gamma/CD172g (CAT# ATGP4030) in a Functional ELISA assay.)
CD47 active protein
Recombinant human CD47 protein
Gene Names
CD47; IAP; OA3; MER6
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
CD47; N/A; Recombinant human CD47 protein; OA3; IAP; MER6; Leukocyte surface antigen CD47 isoform 1; antigenic surface determinant protein OA3; Integrin-associated protein; CD47 active protein
Host
Baculovirus
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
1mg/ml (determined by absorbance at 280nm) (varies by lot)
Sequence Positions
19-141aa
Sequence
QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PNE
Applicable Applications for CD47 active protein
Bioactivity, SDS-PAGE
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Human
Tag
His-Tag
Predicted N-term
Gln19
Bioactivity
Measured by its binding ability in a functional ELISA with Human SIRP gamma/CD172g
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
Bioactivity
(Human CD47 is coated at 1 ug/ml (100 ul/well) can bind Human SIRP gamma/CD172g (CAT# ATGP4030) in a Functional ELISA assay.)
Bioactivity
(Human CD47 is coated at 1 ug/ml (100 ul/well) can bind Human SIRP gamma/CD172g (CAT# ATGP4030) in a Functional ELISA assay.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for CD47 active protein
CD47, also known as leukocyte surface antigen CD47 isoform 1, has a role in both cell adhesions by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Recombinant human CD47, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for CD47 active protein
References
Iwamoto C et al., (2014) Exp. Hematol. 42(3):163-171. Wang Y et al., (2013) Mol. Ther. 21(10):1919-1929.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14.7kDa (129aa) 18-28KDa (SDS-PAGE under reducing conditions.)
NCBI Official Full Name
leukocyte surface antigen CD47 isoform 1
NCBI Official Synonym Full Names
CD47 molecule
NCBI Official Symbol
CD47
NCBI Official Synonym Symbols
IAP; OA3; MER6
NCBI Protein Information
leukocyte surface antigen CD47
UniProt Protein Name
Leukocyte surface antigen CD47
UniProt Gene Name
CD47
UniProt Synonym Gene Names
MER6; IAP
UniProt Entry Name
CD47_HUMAN
Similar Products
Product Notes
The CD47 cd47 (Catalog #AAA48392) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 19-141aa. AAA Biotech's CD47 can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the CD47 cd47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PNEPrecautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.