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product-image-AAA279338_FCM11.jpg FCM/FACS (Flow Cytometry) (Untransfected CT26 cells (green line) and transfected Human DLL3 & CD47 CT26 stable cells (red line) were stained with anti-DLL3 antibody (2ug/1*106cells), washed and then followed by APC-conjugated anti-Human IgG FC/FACS antibody and analyzed with flow cytometry.)

CD47 recombinant antibody

CD47 Recombinant Monoclonal Antibody

Gene Names
CD47; IAP; OA3; MER6
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Affinity chromatography
Synonyms
CD47, Recombinant Antibody; CD47 Recombinant Monoclonal Antibody; CD47; Antigen identified by monoclonal antibody 1D8 antibody; Antigenic surface determinant protein OA3 antibody; CD 47 antibody; CD47 antibody; CD47 antigen (Rh-related antigen; integrin-associated signal transducer) antibody; CD47 antigen antibody; CD47 glycoprotein antibody; CD47 molecule antibody; CD47_HUMAN antibody; IAP antibody; Integrin Associated Protein antibody; Integrin associated signal transducer antibody; Integrin-associated protein antibody; Leukocyte surface antigen CD47 antibody; MER 6 antibody; MER6 antibody; OA 3 antibody; OA3 antibody; OTTHUMP00000041152 antibody; OTTHUMP00000041153 antibody; Protein MER6 antibody; Rh related antigen antibody; Surface antigen identified by monoclonal antibody 1D8 antibody; CD47 recombinant antibody
Ordering
Host
HEK293F Cell
Reactivity
Human
Clonality
Monoclonal
Isotype
hIgG1
Clone Number
4E5
Purity/Purification
Affinity chromatography
Form/Format
Liquid; 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Applicable Applications for CD47 recombinant antibody
FCM/FACS (Flow Cytometry), ELISA
Antigen Species
Human
Immunogen
Recombinant Human CD47 protein
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

FCM/FACS (Flow Cytometry)

(Untransfected CT26 cells (green line) and transfected Human DLL3 & CD47 CT26 stable cells (red line) were stained with anti-DLL3 antibody (2ug/1*106cells), washed and then followed by APC-conjugated anti-Human IgG FC/FACS antibody and analyzed with flow cytometry.)

product-image-AAA279338_FCM11.jpg FCM/FACS (Flow Cytometry) (Untransfected CT26 cells (green line) and transfected Human DLL3 & CD47 CT26 stable cells (red line) were stained with anti-DLL3 antibody (2ug/1*106cells), washed and then followed by APC-conjugated anti-Human IgG FC/FACS antibody and analyzed with flow cytometry.)

FCM/FACS (Flow Cytometry)

(Untransfected CT26 cells (green line) and transfected Human DLL3 & CD47 CT26 stable cells (red line) were stained with anti-CD47 antibody (AAA279338) (2ug/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG FC/FACS antibody and analyzed with flow cytometry.)

product-image-AAA279338_FCM13.jpg FCM/FACS (Flow Cytometry) (Untransfected CT26 cells (green line) and transfected Human DLL3 & CD47 CT26 stable cells (red line) were stained with anti-CD47 antibody (AAA279338) (2ug/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG FC/FACS antibody and analyzed with flow cytometry.)

Activity

(The Binding Activity of Human CD47 with Anti-CD47 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 2ug/mL can bind Anti-CD47 recombinant antibody. The EC50 is 1.343-1.561ng/mL.)

product-image-AAA279338_ACTIVITY15.jpg Activity (The Binding Activity of Human CD47 with Anti-CD47 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 2ug/mL can bind Anti-CD47 recombinant antibody. The EC50 is 1.343-1.561ng/mL.)
Product Categories/Family for CD47 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
961
UniProt Accession #
Molecular Weight
323
NCBI Official Full Name
Leukocyte surface antigen CD47
NCBI Official Synonym Full Names
CD47 molecule
NCBI Official Symbol
CD47
NCBI Official Synonym Symbols
IAP; OA3; MER6
NCBI Protein Information
leukocyte surface antigen CD47; CD47 glycoprotein; Rh-related antigen; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; antigenic surface determinant protein OA3; antigen identified by monoclonal antibody 1D
UniProt Protein Name
Leukocyte surface antigen CD47
UniProt Gene Name
CD47
UniProt Synonym Gene Names
MER6; IAP
UniProt Entry Name
CD47_HUMAN

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Product Notes

The CD47 cd47 (Catalog #AAA279338) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The CD47 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD47 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CD47 cd47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD47, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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