CD47 recombinant protein

Recombinant human CD47 protein

Cat. Num. AAA48419

• Gene Names: CD47; IAP; OA3; MER6
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: CD47; N/A; Recombinant human CD47 protein; Leukocyte surface antigen CD47 isoform 1; IAP; MER6; OA3; CD47 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 19-141aa
• Sequence: QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PNE

• Applicable Applications for CD47 recombinant protein: Bioactivity, SDS-PAGE
• Species: Human
• Tag: hIgG-His-Tag
• Predicted Nterm: Gln19
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Bioactivity: Measured by its binding ability in a functional ELISA with Human SIRP alpha/CD172a. The ED50 range <= 100 ng/ml.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human SIRP alpha/CD172a is coated at 2 ug/ml (100 ul/well) can bind Human CD47. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for CD47 recombinant protein

CD47, also known as leukocyte surface antigen CD47 isoform 1, is a member of immunoglobulin superfamily. It has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Also, it plays an important role in memory formation and synaptic plasticity in the hippocampus. This protein is a receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 mediates cell-cell adhesion, enhances super antigen-dependent T-cell-mediated proliferation and co-stimulates T-cell activation. It may play a role in membrane transport and/or integrin dependent signal transduction and prevent premature elimination of red blood cells. Recombinant human CD47, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CD47 recombinant protein

Immunology; Adhesion & Movement

References

Iwamoto C., et al. (2014) Exp. Hematol. 42:163-171.;; Wang Y., et al. (2013) Mol. Ther. 21:1919-1929.;; Jiang H., et al. (2013) Leuk. Res. 37:907-910.

NCBI and Uniprot Product Information

• NCBI GI #: 4502673
• NCBI GeneID: 961
• NCBI Accession #: NP_001768
• NCBI GenBank Nucleotide #: NM_001777.3
• UniProt Accession #: Q08722; Q53Y71; Q96A60; A8K198; D3DN59
• Molecular Weight: 14.7kDa (129aa) 18-28KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: leukocyte surface antigen CD47 isoform 1
• NCBI Official Synonym Full Names: CD47 molecule
• NCBI Official Symbol: CD47
• NCBI Official Synonym Symbols: IAP; OA3; MER6
• NCBI Protein Information: leukocyte surface antigen CD47
• UniProt Protein Name: Leukocyte surface antigen CD47
• UniProt Gene Name: CD47
• UniProt Synonym Gene Names: MER6; IAP
• UniProt Entry Name: CD47_HUMAN