Interleukin-17A (IL17A)(T26A) Active Protein | IL17A active protein

Recombinant Human Interleukin-17A (IL17A)(T26A)(Active)

Cat. Num. AAA279249

• Gene Names: IL17A; IL17; CTLA8; IL-17; IL-17A
• Purity: Greater than 95% as determined by SDS-PAGE.
• Synonyms: Interleukin-17A (IL17A)(T26A); N/A; Recombinant Human Interleukin-17A (IL17A)(T26A)(Active); IL-17; IL-17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8; IL17A active protein

• Host: Baculovirus
• Purity/Purification: Greater than 95% as determined by SDS-PAGE.
• Form/Format: Lyophilized powder; Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
• Sequence Positions: 24-155aa (T26A); Full Length of Mature Protein
• Sequence: GIAIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA$

• Species: Homo sapiens (Human)
• Tag: N-terminal 6xHis-tagged
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Research Area: Immunology
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human IL17A at 2 ug/ml can bind Anti-IL17A recombinant antibody, the EC50 is 1.818-2.170 ng/mL.
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.; Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.; We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Bioactivity

(Measured by its binding ability in a functional ELISA. Immobilized Human IL17A at 2 ?g/ml can bind Anti-IL17A recombinant antibody, the EC50 is 1.818-2.170 ng/mL.)

SDS-PAGE

((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)

Related Product Information for IL17A active protein

Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines. In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance. Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation. Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection. Upon acute injury, has a direct role in epithelial barrier formation by regulating OCLN localization and tight junction biogenesis. As part of the mucosal immune response induced by commensal bacteria, enhances host's ability to resist pathogenic bacterial and fungal infections by promoting neutrophil recruitment and antimicrobial peptides release. In synergy with IL17F, mediates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers. Involved in antiviral host defense through various mechanisms. Enhances immunity against West Nile virus by promoting T cell cytotoxicity. May play a beneficial role in influenza A virus (H5N1) infection by enhancing B cell recruitment and immune response in the lung. Contributes to influenza A virus (H1N1) clearance by driving the differentiation of B-1a B cells, providing for production of virus-specific IgM antibodies at first line of host defense.

Product Categories/Family for IL17A active protein

Active Protein & In Stock Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4504651
• NCBI GeneID: 3605
• NCBI Accession #: NP_002181.1
• NCBI GenBank Nucleotide #: NM_002190.2
• UniProt Accession #: Q16552; Q5T2P0
• Molecular Weight: 17,504 Da
• NCBI Official Full Name: interleukin-17A
• NCBI Official Synonym Full Names: interleukin 17A
• NCBI Official Symbol: IL17A
• NCBI Official Synonym Symbols: IL17; CTLA8; IL-17; IL-17A
• NCBI Protein Information: interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• UniProt Protein Name: Interleukin-17A
• UniProt Gene Name: IL17A
• UniProt Synonym Gene Names: CTLA8; IL17; IL-17; IL-17A; CTLA-8
• UniProt Entry Name: IL17_HUMAN