Tie-2 active protein

Recombinant human Tie-2 protein

Cat. Num. AAA48363

• Gene Names: TEK; TIE2; VMCM; GLC3E; TIE-2; VMCM1; CD202B
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Tie-2; N/A; Recombinant human Tie-2 protein; Angiopoietin-1 receptor isoform 1; TEK; CD202B; GLC3E; TIE-2; TIE2; VMCM; VMCM1; ATGP3762; Tie-2 active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 23-748aa
• Sequence: AMDLILINSL PLVSDAETSL TCIASGWRPH EPITIGRDFE ALMNQHQDPL EVTQDVTREW AKKVVWKREK ASKINGAYFC EGRVRGEAIR IRTMKMRQQA SFLPATLTMT VDKGDNVNIS FKKVLIKEED AVIYKNGSFI HSVPRHEVPD ILEVHLPHAQ PQDAGVYSAR YIGGNLFTSA FTRLIVRRCE AQKWGPECNH LCTACMNNGV CHEDTGECIC PPGFMGRTCE KACELHTFGR TCKERCSGQE GCKSYVFCLP DPYGCSCATG WKGLQCNEAC HPGFYGPDCK LRCSCNNGEM CDRFQGCLCS PGWQGLQCER EGIPRMTPKI VDLPDHIEVN SGKFNPICKA SGWPLPTNEE MTLVKPDGTV LHPKDFNHTD HFSVAIFTIH RILPPDSGVW VCSVNTVAGM VEKPFNISVK VLPKPLNAPN VIDTGHNFAV INISSEPYFG DGPIKSKKLL YKPVNHYEAW QHIQVTNEIV TLNYLEPRTE YELCVQLVRR GEGGEGHPGP VRRFTTASIG LPPPRGLNLL PKSQTTLNLT WQPIFPSSED DFYVEVERRS VQKSDQQNIK VPGNLTSVLL NNLHPREQYV VRARVNTKAQ GEWSEDLTAW TLSDILPPQP ENIKISNITH SSAVISWTIL DGYSISSITI RYKVQGKNED QHVDVKIKNA TITQYQLKGL EPETAYQVDI FAENNIGSSN PAFSHELVTL PESQAPADLG GGKMLL

• Applicable Applications for Tie-2 active protein: Bioactivity, SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: hIgG-His-Tag
• Predicted N-term: Ala23
• Bioactivity: Measured by the ability of the binding activity in a functional ELISA. The ED50 range <=1ug/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human Angiopoietin-2 is coated at 5 ug/ml (100 ul/well) can bind Human Tie-2. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for Tie-2 active protein

TEK, also known as angiopoietin-1 receptor isoform 1, is an endothelial cell-specific receptor tyrosine kinase subfamily (RTK). This protein acts as cell-surface receptor and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton. It has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels and is required for normal angiogenesis and heart development during embryogenesis. Recombinant human TEK protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Tie-2 active protein

Signal Transduction; Proteins

References

Kitajima D., et al, (2016) J Cancer. 7:600-607. Kelly-Goss MR., et al, (2017) Sci Rep. 7:9049.

NCBI and Uniprot Product Information

• NCBI GI #: 88758596
• NCBI GeneID: 7010
• NCBI Accession #: NP_000450
• NCBI GenBank Nucleotide #: NM_000459.4
• UniProt Accession #: Q02763; Q5TCU2; Q8IV34; A8K6W0; B4DH20; B4DHD3; D3DRK5; E7EWI2
• Molecular Weight: 107.9kDa (965aa) 100-150KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: angiopoietin-1 receptor isoform 1
• NCBI Official Synonym Full Names: TEK receptor tyrosine kinase
• NCBI Official Symbol: TEK
• NCBI Official Synonym Symbols: TIE2; VMCM; GLC3E; TIE-2; VMCM1; CD202B
• NCBI Protein Information: angiopoietin-1 receptor
• UniProt Protein Name: Angiopoietin-1 receptor
• UniProt Gene Name: TEK
• UniProt Synonym Gene Names: TIE2; VMCM; VMCM1; hTIE2
• UniProt Entry Name: TIE2_HUMAN