Human Livin ELISA Kit | BIRC7 elisa kit

Human Livin ELISA Kit

Cat. Num. AAA261805

• Gene Names: BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• Reactivity: Human
• Applications: ELISA
• Synonyms: Livin; N/A; Human Livin ELISA Kit; KIAP; LIVIN; MLIAP; RNF50; Baculoviral IAP repeat-containing protein 7; Kidney inhibitor of apoptosis protein; Melanoma inhibitor of apoptosis protein; ML-IAP; RING finger protein 50; BIRC7 elisa kit

• Reactivity: Human
• Specificity: The Human Livin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Livin proteins within the range of 780 pg/ml - 50000 pg/ml.
• Sequence Length: 298
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 20 pg/ml.

• Applicable Applications for BIRC7 elisa kit: ELISA (Sandwich ELISA)
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for BIRC7 elisa kit

Principle of the Assay||The Human Livin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Livin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Livin coated on a 96-well plate. Standards and samples are pipetted into the wells and Livin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Livin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Livin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: BIRC7, also called KIAP or Livin, is a protein that in humans is encoded by the BIRC7 gene. This gene is mapped to 20q13.33. It encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. BIRC7 blocked apoptosis induced by BAX induction of cytochrome C release from mitochondria, and it also can block apoptosis induced by chemotherapeutic agents.

NCBI and Uniprot Product Information

• NCBI GI #: 21759008
• NCBI GeneID: 79444
• UniProt Accession #: Q96CA5; Q9BQV0; Q9H2A8; Q9HAP7
• Molecular Weight: 30,866 Da
• NCBI Official Full Name: Baculoviral IAP repeat-containing protein 7
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 7
• NCBI Official Symbol: BIRC7
• NCBI Official Synonym Symbols: KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• NCBI Protein Information: baculoviral IAP repeat-containing protein 7
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 7
• UniProt Gene Name: BIRC7
• UniProt Synonym Gene Names: KIAP; LIVIN; MLIAP; RNF50; KIAP; ML-IAP; Truncated livin; p30-Livin; tLivin
• UniProt Entry Name: BIRC7_HUMAN