Rabbit anti-Human BIRC7 / Livin Polyclonal Antibody | anti-BIRC7 antibody

Rabbit Polyclonal to Human BIRC7 / Livin

Cat. Num. AAA50732

• Gene Names: BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Peptide Column Affinity Chromatography
• Synonyms: BIRC7 / Livin, Antibody; Rabbit Polyclonal to Human BIRC7 / Livin; Anti-BIRC7 / Livin Antibody (aa264-280) IHC-plus; BIRC7; KIAP; Livin inhibitor of apoptosis; MLIAP; ML-IAP; RNF50; RING finger protein 50; LIVIN; Human BIRC7; Livin; anti-BIRC7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: A lower but much weaker band at 30 kDa was detected in Raji cell lysate, which may represent the short form of Livin.
• Purity/Purification: Peptide Column Affinity Chromatography
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 280

• Applicable Applications for anti-BIRC7 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human BIRC7 / Livin. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Rabbit (93%); Marmoset, Panda, Bovine (86%).
• Family/Subfamily: Apoptosis
• Antigen type: Synthetic Peptide
• Immunogen Species: BIRC7 / Livin antibody was raised agaisnt human
• Immunogen: BIRC7 / Livin antibody was raised agasint 17 AA peptide near the C-Terminus of human livin. The immunogen is located within the last 50 AA of livin
• Antigen Modification: aa264-280
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

WB (Western Blot)

(Western blot of Livin expression in human Raji cell lysate with Livin antibody at 0.5 ug/ml.)

IF (Immunofluorescence)

(Immunofluorescence of Livin in Human Small Intestine cells with Livin antibody at 20 ug/ml.)

IHC (Immunohistochemistry)

(Anti-BIRC7 / Livin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-BIRC7 antibody

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP.

Product Categories/Family for anti-BIRC7 antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 11545910
• NCBI GeneID: 79444
• NCBI Accession #: NP_071444.1
• NCBI GenBank Nucleotide #: NM_022161.3
• UniProt Accession #: Q96CA5; Q9BQV0; Q9H2A8; Q9HAP7
• Molecular Weight: 30,866 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 7 isoform beta
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 7
• NCBI Official Symbol: BIRC7
• NCBI Official Synonym Symbols: KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• NCBI Protein Information: baculoviral IAP repeat-containing protein 7; RING finger protein 50; livin inhibitor of apoptosis; kidney inhibitor of apoptosis protein; melanoma inhibitor of apoptosis protein
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 7
• UniProt Gene Name: BIRC7
• UniProt Synonym Gene Names: KIAP; LIVIN; MLIAP; RNF50; KIAP; ML-IAP; Truncated livin; p30-Livin; tLivin
• UniProt Entry Name: BIRC7_HUMAN