Bovine Probable ATP-dependent RNA helicase DDX58 (DDX58) ELISA Kit | DDX58 elisa kit

Bovine Probable ATP-dependent RNA helicase DDX58 (DDX58) ELISA Kit

Cat. Num. AAA245440

• Gene Names: DDX58; RIGI; RIG-I; RLR-1
• Reactivity: Bovine
• Synonyms: Probable ATP-dependent RNA helicase DDX58 (DDX58); N/A; Bovine Probable ATP-dependent RNA helicase DDX58 (DDX58) ELISA Kit; DDX58 elisa kit

• Reactivity: Bovine
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for DDX58 elisa kit

Intended Uses: This DDX58 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine DDX58.

Principle of the Assay: DDX58 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DDX58 antibody and an DDX58-HRP conjugate. The assay sample and buffer are incubated together with DDX58-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDX58 concentration since DDX58 from samples and DDX58-HRP conjugate compete for the anti-DDX58 antibody binding site. Since the number of sites is limited, as more sites are occupied by DDX58 from the sample, fewer sites are left to bind DDX58-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDX58 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DDX58 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 27881482
• NCBI GeneID: 23586
• NCBI Accession #: NP_055129.2
• NCBI GenBank Nucleotide #: NM_014314.3
• Molecular Weight: 101,377 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX58
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• NCBI Official Symbol: DDX58
• NCBI Official Synonym Symbols: RIGI; RIG-I; RLR-1
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX58; RIG-1; RNA helicase RIG-I; DEAD box protein 58; RIG-I-like receptor 1; retinoic acid inducible gene I; retinoic acid-inducible gene 1 protein; retinoic acid-inducible gene I protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX58
• UniProt Gene Name: DDX58
• UniProt Synonym Gene Names: RLR-1; RIG-1; RIG-I
• UniProt Entry Name: DDX58_HUMAN