Application Data
(HTRF Assay for Recombinant BRD4 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD4 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF was used to detect the products.)
BRD4 recombinant protein
Recombinant BRD4 protein
Purity
>=95% by SDS-PAGE.
Synonyms
BRD4; N/A; Recombinant BRD4 protein; Bromodomain containing protein 4; HUNK1; FSHRG4; HUNKI; MCAP; CAP; BRD4 recombinant protein
Host
E coli
Purity/Purification
>=95% by SDS-PAGE.
Form/Format
Recombinant BRD4 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant BRD4 protein that includes full length of human BRD4 protein (accession number NP_055114.1) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with a molecular weight of 81.7kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(HTRF Assay for Recombinant BRD4 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD4 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF was used to detect the products.)
Application Data
(HTRF Assay for Recombinant BRD4 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD4 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF was used to detect the products.)
Application Data
(HTRF Assay for Recombinant BRD4 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD4 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF was used to detect the products.)
Application Data
(HTRF Assay for Recombinant BRD4 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD4 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF was used to detect the products.)
SDS-PAGE
(Recombinant BRD4 protein gel 10% SDS-PAGE Coomassie staining MW: 81.7kDa Purity: >95%)
SDS-PAGE
(Recombinant BRD4 protein gel 10% SDS-PAGE Coomassie staining MW: 81.7kDa Purity: >95%)
Related Product Information for BRD4 recombinant protein
Bromodomain-containing protein 4 (BRD4) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female sterile homeotic protein gene in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD4 has been identified recently as a therapeutic target in many cancers, including acute myeloid leukemia, multiple myeloma, Burkitt’s lymphoma, NUT midline carcinoma, colon cancer, and breast cancer. BRD4 regulates the transcription of oncogenes, HIV, and human papilloma virus (HPV). It has been shown to bind and phosphorylate RNA pol II, which implicates its involvement in the regulation of eukaryotic transcription. It shows binding specificity for acetylated H3K9, H3K9/K14, H4K5, H4K8, H4K12, H4K5/K8, H4K5/K12, H4K8/K12, H4K12/K16, H4K12/K16/K20 and H4K5/K8/K12/K16, as well as acetylated RelA-K310.
Product Categories/Family for BRD4 recombinant protein
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Product Notes
The BRD4 (Catalog #AAA60413) is a Recombinant Protein produced from E coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BRD4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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