Mouse Bromodomain-containing protein 4 (BRD4) ELISA Kit | BRD4 elisa kit

Mouse Bromodomain-containing protein 4 (BRD4) ELISA Kit

Cat. Num. AAA27362

• Gene Names: BRD4; CAP; MCAP; HUNK1; HUNKI
• Reactivity: Mouse
• Synonyms: Bromodomain-containing protein 4 (BRD4); Mouse Bromodomain-containing protein 4 (BRD4) ELISA Kit; BRD4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of BRD4. No significant cross-reactivity or interference between BRD4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BRD4 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for BRD4 elisa kit

Principle of the Assay: BRD4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BRD4 antibody and an BRD4-HRP conjugate. The assay sample and buffer are incubated together with BRD4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BRD4 concentration since BRD4 from samples and BRD4-HRP conjugate compete for the anti-BRD4 antibody binding site. Since the number of sites is limited, as more sites are occupied by BRD4 from the sample, fewer sites are left to bind BRD4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BRD4 concentration in each sample is interpolated from this standard curve.

Intended Uses: This BRD4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BRD4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for BRD4 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 19718731
• NCBI GeneID: 23476
• NCBI Accession #: NP_490597.1
• NCBI GenBank Nucleotide #: NM_058243.2
• Molecular Weight: 88,289 Da
• NCBI Official Full Name: bromodomain-containing protein 4 isoform long
• NCBI Official Synonym Full Names: bromodomain containing 4
• NCBI Official Symbol: BRD4
• NCBI Official Synonym Symbols: CAP; MCAP; HUNK1; HUNKI
• NCBI Protein Information: bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein
• UniProt Protein Name: Bromodomain-containing protein 4
• UniProt Gene Name: BRD4
• UniProt Synonym Gene Names: HUNK1
• UniProt Entry Name: BRD4_HUMAN