IF (Immunofluorescence)
(Immunofluorescent analysis of BRD4 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
BRD4 recombinant antibody
Recombinant Anti-BRD4 Rabbit mAb
Gene Names
BRD4; CAP; MCAP; HUNK1; HUNKI
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
BRD4, Recombinant Antibody; Recombinant Anti-BRD4 Rabbit mAb; HUNK1; Bromodomain-containing protein 4; Protein HUNK1; BRD4 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Specificity
Recognizes endogenous levels of BRD4 protein
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in PBS, pH7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Applicable Applications for BRD4 recombinant antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human BRD4. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of BRD4 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of BRD4 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot)
(Western blot analysis of BRD4 expression in HepG2 (A) whole cell lysates. (Predicted band size: 152 kD; Observed band size: 152 kD))
WB (Western Blot)
(Western blot analysis of BRD4 expression in HepG2 (A) whole cell lysates. (Predicted band size: 152 kD; Observed band size: 152 kD))
Related Product Information for BRD4 recombinant antibody
Recombinant rabbit monoclonal antibody to BRD4
Product Categories/Family for BRD4 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
152kDa
NCBI Official Full Name
bromodomain-containing protein 4 isoform short
NCBI Official Synonym Full Names
bromodomain containing 4
NCBI Official Symbol
BRD4
NCBI Official Synonym Symbols
CAP; MCAP; HUNK1; HUNKI
NCBI Protein Information
bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein
UniProt Protein Name
Bromodomain-containing protein 4
UniProt Gene Name
BRD4
UniProt Synonym Gene Names
HUNK1
UniProt Entry Name
BRD4_HUMAN
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Product Notes
The BRD4 brd4 (Catalog #AAA266371) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-BRD4 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRD4 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the BRD4 brd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRD4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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