Human complement C5a ELISA Kit | C5 elisa kit

Human complement C5a PicoKine ELISA Kit

Cat. Num. AAA45847

• Gene Names: C5; C5D; C5a; C5b; ECLZB; CPAMD4
• Reactivity: Human
• Synonyms: complement C5a; N/A; Human complement C5a PicoKine ELISA Kit; Complement C5; complement component 5; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C5; CO5_HUMAN; complement C5; Complement C5 alpha chain; Complement component 5; CPAMD4; C5 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human complement C5a
• Sequence Length: 1676
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human complement C5a PicoKine ELISA Kit standard curve)

Related Product Information for C5 elisa kit

Principle of the assay: human complement C5a ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for complement C5a has been precoated onto 96-well plates. Standards(E.coli, T678-R751) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for complement C5a is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human complement C5a amount of sample captured in plate.
Background: C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

NCBI and Uniprot Product Information

• NCBI GI #: 166900096
• NCBI GeneID: 727
• UniProt Accession #: P01031; Q14CJ0; Q27I61
• Molecular Weight: 188,305 Da
• NCBI Official Full Name: Complement C5
• NCBI Official Synonym Full Names: complement component 5
• NCBI Official Symbol: C5
• NCBI Official Synonym Symbols: C5D; C5a; C5b; ECLZB; CPAMD4
• NCBI Protein Information: complement C5; prepro-C5; C5a anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
• UniProt Protein Name: Complement C5
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: CPAMD4
• UniProt Entry Name: CO5_HUMAN