WB (Western Blot)
(Recombinant PRMT4 (CARM1) protein activity assay 0.5 ug Histone H3.1 were incubated with 0, 0.05 ug, 0.1 ug, 0.2 ug PRMT4 (CARM1) in 30 ul reaction system for 2 hours at room temperature, respectively. 6 ul reaction samples were loaded and run on a 13% SDS-PAGE gel. Western blot was used to detect the generation of products (H3R17me2a antibody). The western blot results show that histone H3 is di-methylated on its Arginine 17 by PRMT4 (CARM1).)
PRMT4 recombinant protein
Recombinant PRMT4 (CARM1) protein
Gene Names
CARM1; PRMT4
Synonyms
PRMT4; N/A; Recombinant PRMT4 (CARM1) protein; Coactivator Associated Arginine Methyltransferase 1; Protein Arginine N-Methyltransferase 4; PRMT4 recombinant protein
Host
Baculovirus
Form/Format
Full length recombinant PRMT4 (CARM1) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant PRMT4 (CARM1) protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
WB (Western Blot)
(Recombinant PRMT4 (CARM1) protein activity assay 0.5 ug Histone H3.1 were incubated with 0, 0.05 ug, 0.1 ug, 0.2 ug PRMT4 (CARM1) in 30 ul reaction system for 2 hours at room temperature, respectively. 6 ul reaction samples were loaded and run on a 13% SDS-PAGE gel. Western blot was used to detect the generation of products (H3R17me2a antibody). The western blot results show that histone H3 is di-methylated on its Arginine 17 by PRMT4 (CARM1).)
WB (Western Blot)
(Recombinant PRMT4 (CARM1) protein activity assay 0.5 ug Histone H3.1 were incubated with 0, 0.05 ug, 0.1 ug, 0.2 ug PRMT4 (CARM1) in 30 ul reaction system for 2 hours at room temperature, respectively. 6 ul reaction samples were loaded and run on a 13% SDS-PAGE gel. Western blot was used to detect the generation of products (H3R17me2a antibody). The western blot results show that histone H3 is di-methylated on its Arginine 17 by PRMT4 (CARM1).)
SDS-PAGE
(Recombinant PRMT4 (CARM1) protein gel 8% SDS-PAGE Coomassie staining MW: 66.8 kDa Purity: >88% )
SDS-PAGE
(Recombinant PRMT4 (CARM1) protein gel 8% SDS-PAGE Coomassie staining MW: 66.8 kDa Purity: >88% )
Related Product Information for PRMT4 recombinant protein
Short Description: Recombinant human PRMT4 (CARM1) was expressed in a baculovirus expression system as the full-length protein (accession number NP_954592.1) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 66.8 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: PRMT4 (CARM1)(Coactivator-associated arginine methyltransferase 1 or Carm1) is a protein arginine N-methyltransferase that catalyzes the mono- and asymmetric dimethylation of arginine residues in its substrate proteins. It serves as a coactivator of transcription and also plays a role in nuclear receptors. PRMT4 (CARM1) methylates histone H3 at Arg2, Arg17 and Arg26; it also methylates a number of non-histone substrates involved in the regulation and mechanism of gene expression.
Background: PRMT4 (CARM1)(Coactivator-associated arginine methyltransferase 1 or Carm1) is a protein arginine N-methyltransferase that catalyzes the mono- and asymmetric dimethylation of arginine residues in its substrate proteins. It serves as a coactivator of transcription and also plays a role in nuclear receptors. PRMT4 (CARM1) methylates histone H3 at Arg2, Arg17 and Arg26; it also methylates a number of non-histone substrates involved in the regulation and mechanism of gene expression.
Product Categories/Family for PRMT4 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,885 Da
NCBI Official Full Name
histone-arginine methyltransferase CARM1
NCBI Official Synonym Full Names
coactivator-associated arginine methyltransferase 1
NCBI Official Symbol
CARM1
NCBI Official Synonym Symbols
PRMT4
NCBI Protein Information
histone-arginine methyltransferase CARM1; protein arginine N-methyltransferase 4
UniProt Protein Name
Histone-arginine methyltransferase CARM1
UniProt Gene Name
CARM1
UniProt Synonym Gene Names
PRMT4
UniProt Entry Name
CARM1_HUMAN
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Product Notes
The PRMT4 carm1 (Catalog #AAA60138) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PRMT4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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