FCM/FACS (Flow Cytometry)
(PRMT4 Antibody(Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit PRMT4 Polyclonal Antibody | anti-CARM1 antibody
PRMT4 Antibody (Center)
Gene Names
CARM1; PRMT4
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PRMT4, Antibody; PRMT4 Antibody (Center); Histone-arginine methyltransferase CARM1; 211-; Coactivator-associated arginine methyltransferase 1; Protein arginine N-methyltransferase 4; CARM1; PRMT4; anti-CARM1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PRMT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-377 amino acids from the Central region of human PRMT4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
346-377
Sequence Length
384
Applicable Applications for anti-CARM1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PRMT4 Antibody(Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PRMT4 Antibody(Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(PRMT4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRMT4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PRMT4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRMT4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PRMT4 Antibody(Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PRMT4 antibody detected the PRMT4 protein (arrow).)
WB (Western Blot)
(PRMT4 Antibody(Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PRMT4 antibody detected the PRMT4 protein (arrow).)
Related Product Information for anti-CARM1 antibody
Protein arginine N-methyltransferases, such as CARM1,
catalyze the transfer of a methyl group from
S-adenosyl-L-methionine to the side chain nitrogens of arginine
residues within proteins to form methylated arginine derivatives
and S-adenosyl-L-homocysteine. Protein arginine methylation has
been implicated in signal transduction, metabolism of nascent
pre-RNA, and transcriptional activation (Frankel et al., 2002
[PubMed 11724789]).
catalyze the transfer of a methyl group from
S-adenosyl-L-methionine to the side chain nitrogens of arginine
residues within proteins to form methylated arginine derivatives
and S-adenosyl-L-homocysteine. Protein arginine methylation has
been implicated in signal transduction, metabolism of nascent
pre-RNA, and transcriptional activation (Frankel et al., 2002
[PubMed 11724789]).
Product Categories/Family for anti-CARM1 antibody
References
Gao, X., et al. J. Cell. Biochem. 110(1):162-170(2010)
Carascossa, S., et al. Genes Dev. 24(7):708-719(2010)
Kim, Y.R., et al. BMC Cancer 10, 197 (2010) :
Ito, T., et al. BMC Dev. Biol. 9, 47 (2009) :
Haiman, C.A., et al. BMC Cancer 9, 43 (2009) :
Carascossa, S., et al. Genes Dev. 24(7):708-719(2010)
Kim, Y.R., et al. BMC Cancer 10, 197 (2010) :
Ito, T., et al. BMC Dev. Biol. 9, 47 (2009) :
Haiman, C.A., et al. BMC Cancer 9, 43 (2009) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65854
NCBI Official Full Name
histone-arginine methyltransferase CARM1
NCBI Official Synonym Full Names
coactivator-associated arginine methyltransferase 1
NCBI Official Symbol
CARM1
NCBI Official Synonym Symbols
PRMT4
NCBI Protein Information
histone-arginine methyltransferase CARM1
UniProt Protein Name
Histone-arginine methyltransferase CARM1
UniProt Gene Name
CARM1
UniProt Synonym Gene Names
PRMT4
UniProt Entry Name
CARM1_HUMAN
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Product Notes
The CARM1 carm1 (Catalog #AAA284794) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 346-377. The PRMT4 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PRMT4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CARM1 carm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRMT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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