IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PRMT4 antibody (C-term)(Cat.#AP1006a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human PRMT4 Polyclonal Antibody | anti-PRMT4 antibody
PRMT4 Antibody (C-term)
Gene Names
CARM1; PRMT4
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Synonyms
PRMT4, Antibody; PRMT4 Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab); Histone-arginine methyltransferase CARM1; 211-; Coactivator-associated arginine methyltransferase 1; Protein arginine N-methyltransferase 4; CARM1; PRMT4; anti-PRMT4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB0032
Specificity
This PRMT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PRMT4.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applicable Applications for anti-PRMT4 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PRMT4 antibody (C-term)(Cat.#AP1006a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PRMT4 antibody (C-term)(Cat.#AP1006a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of PRMT4 polyclonal antibody in whole HL60 cell lysate: PRMT4 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
WB (Western Blot)
(Western blot analysis of PRMT4 polyclonal antibody in whole HL60 cell lysate: PRMT4 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Related Product Information for anti-PRMT4 antibody
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
Product Categories/Family for anti-PRMT4 antibody
References
Wada K, et al. Biochim Biophys Acta. 2002. 1591:1.Cimato TR, et al. J Neurosci Res. 2002. 67:435.Frankel A, et al. J Biol Chem. 2002. 277:3537.Brahms H, et al. RNA. 2001. 7:1531.Pelletier M, et al. Mol Biochem Parasitol. 2001. 118:49.Belyanskaya LL, et al. J Biol Chem. 2001. 276:18681.Rho J, et al. J Biol Chem. 2001. 276:11393.Scorilas A, et al. Biochem Biophys Res Commun. 2000. 278:349.Frankel A, et al. J Biol Chem. 2000. 275:32974.Zhang X, et al. EMBO J. 19:3509.Tang J, et al. J Biol Chem. 1998. 273:16935.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,885 Da
NCBI Official Full Name
histone-arginine methyltransferase CARM1
NCBI Official Synonym Full Names
coactivator-associated arginine methyltransferase 1
NCBI Official Symbol
CARM1
NCBI Official Synonym Symbols
PRMT4
NCBI Protein Information
histone-arginine methyltransferase CARM1; protein arginine N-methyltransferase 4
UniProt Protein Name
Histone-arginine methyltransferase CARM1
UniProt Gene Name
CARM1
UniProt Synonym Gene Names
PRMT4
UniProt Entry Name
CARM1_HUMAN
Similar Products
Product Notes
The PRMT4 carm1 (Catalog #AAA290967) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRMT4 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRMT4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PRMT4 carm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRMT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.