Human caveolin 1, caveolae protein, 22kDa ELISA Kit | CAV1 elisa kit

Human Caveolin-1, Cav-1 ELISA Kit

Cat. Num. AAA80531

• Gene Names: CAV1; CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
• Reactivity: Human
• Synonyms: caveolin 1, caveolae protein, 22kDa; N/A; Human Caveolin-1, Cav-1 ELISA Kit; Human Caveolin-1; Cav-1 ELISA KIT; BSCL3; CAV; CGL3; MSTP085; VIP21; caveolae protein; 22-kD; caveolin 1; alpha isoform; beta isoform; cell growth-inhibiting protein 32; 22kDa; CAV1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CAV1. No significant cross-reactivity or interference between Human CAV1 and analogues was observed.
• Sequence Length: 178
• Samples: Serum, plasma, tissue homogenates
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: 7.8 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 3: Host-virus interaction
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for CAV1 elisa kit

Introduction: Caveolin-1 is a protein that in humans is encoded by the CAV1 gene. The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cav-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cav-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Cav-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cav-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15451856
• NCBI GeneID: 857
• NCBI Accession #: NP_001744.2
• NCBI GenBank Nucleotide #: NM_001753.4
• UniProt Accession #: Q03135; Q9UGP1; Q9UNG1; Q9UQH6
• Molecular Weight: 20,472 Da
• NCBI Official Full Name: caveolin-1 isoform alpha
• NCBI Official Synonym Full Names: caveolin 1, caveolae protein, 22kDa
• NCBI Official Symbol: CAV1
• NCBI Official Synonym Symbols: CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
• NCBI Protein Information: caveolin-1; cell growth-inhibiting protein 32
• UniProt Protein Name: Caveolin-1
• UniProt Gene Name: CAV1
• UniProt Synonym Gene Names: CAV
• UniProt Entry Name: CAV1_HUMAN