CCL2/JE/MCP-1 recombinant protein

Recombinant human CCL2/JE/MCP-1 protein

Cat. Num. AAA48416

• Gene Names: CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CCL2/JE/MCP-1; N/A; Recombinant human CCL2/JE/MCP-1 protein; C-C motif chemokine 2; CCL2; GDCF-2; HSMCR30; HC11; JE; MCAF; MCP-1; MCP1; Scya2; Sigje; SMC-CF; Immediate-early serum-responsive protein JE; Monocyte chemoattractant protein 1; Small-inducible cytokine A2; CCL2/JE/MCP-1 recombinant protein

• Host: HEK293
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 24-99aa
• Sequence: QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

• Applicable Applications for CCL2/JE/MCP-1 recombinant protein: Bioactivity, SDS-PAGE
• Species: Human
• Tag: His-Tag
• Predicted Nterm: Gln 24
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Bioactivity: Measured its ability to chemoattract using THP-1 human acute monocytic leukemia cells. The ED50 range <= 30 ng/ml.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human CCL2/MCP-1 chemoattracts the the THP-1 human acute monocytic leukemia cells. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for CCL2/JE/MCP-1 recombinant protein

CCL2 (Chemokine ligand 2), as known as MCP-1(monocyte chemotactic protein-1), is a small cytokine belonging to the CC chemokine family. This protein recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. It binds to chemokine receptors CCR2 and CCR4. It also induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils, primarily due to interaction with CCR2 receptors on these cells. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. Recombinant human CCL2/JE/MCP-1, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Categories/Family for CCL2/JE/MCP-1 recombinant protein

Immunology; Hormones & Cytokines

References

Foresti ML, Arisi GM, et al. (2009). J Neuroinflammation. 6:40.;; Semple BD, Bye N, et al. (2010). J Cereb Blood Flow Metab. 30:769-782.

NCBI and Uniprot Product Information

• NCBI GI #: 4506841
• NCBI GeneID: 6347
• NCBI Accession #: NP_002973.1
• NCBI GenBank Nucleotide #: NM_002982.3
• UniProt Accession #: P13500; Q9UDF3; B2R4V3
• Molecular Weight: 11kDa
• NCBI Official Full Name: C-C motif chemokine 2
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 2
• NCBI Official Symbol: CCL2
• NCBI Official Synonym Symbols: HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• NCBI Protein Information: C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine
• UniProt Protein Name: C-C motif chemokine 2
• UniProt Gene Name: CCL2
• UniProt Synonym Gene Names: MCP1; SCYA2; MCAF; MCP-1
• UniProt Entry Name: CCL2_HUMAN